Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,882,139	A→G	E17G (GAG→GGG)	tnaA →	tryptophanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,882,139	0	A	G	100.0%	147.6 / NA	40	E17G (GAG→GGG)	tnaA	tryptophanase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/15);  total (25/15)

TATATATCTGGCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCGGGGCGGTGACGCAGA  >  NZ_CP009273/3882008‑3882273                                                                                                                                    |                                                                                                                                       tatatatCTGGCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTa                                                                                                                                 <  2:369552/139‑1 (MQ=255)       tCTGGCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACg                                                                                                                            <  2:20477/138‑1 (MQ=255)       tCTGGCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACg                                                                                                                            <  1:293362/138‑1 (MQ=255)         tGGCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTAc                                                                                                                         >  2:189401/1‑139 (MQ=255)           gCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTAc                                                                                                                         <  2:267371/137‑1 (MQ=255)            cGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCAc                                                                                                                      >  1:51369/1‑139 (MQ=255)                  aaTCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTcgcg                                                                                                                 <  2:426410/138‑1 (MQ=255)                             cAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTg                                                                                                        >  2:136507/1‑136 (MQ=255)                                  gTAATATTCACAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGc                                                                                                 >  1:337232/1‑138 (MQ=255)                                  gTAATATTCACAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGc                                                                                                 >  1:402588/1‑138 (MQ=255)                                   tAATATTCACAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCa                                                                                                <  2:177230/138‑1 (MQ=255)                                          cacaGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAAttatta                                                                                         >  1:126545/1‑138 (MQ=255)                                          cacaGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAAttatta                                                                                         >  2:182801/1‑138 (MQ=255)                                           acaGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTaaa                                                                                       >  2:56034/1‑139 (MQ=255)                                              gggATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATcc                                                                                    <  1:182801/139‑1 (MQ=255)                                                gATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCgg                                                                                  >  1:96537/1‑139 (MQ=255)                                                 aTCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCgg                                                                                  >  1:292006/1‑138 (MQ=255)                                                  tCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTa                                                                                >  2:422816/1‑139 (MQ=255)                                                        tAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGaa                                                                            <  1:420691/137‑1 (MQ=255)                                                             aaaaTAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTc                                                                     >  1:29629/1‑139 (MQ=255)                                                                                 tAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATg                                                  <  2:51369/138‑1 (MQ=255)                                                                                    tGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGtttt                                              >  1:289560/1‑139 (MQ=255)                                                                                      gAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTa                                            >  2:65444/1‑139 (MQ=255)                                                                                         aaCTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATc                                          >  2:270729/1‑138 (MQ=255)                                                                                              tAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTa                                    >  1:313223/1‑139 (MQ=255)                                                                                               aaaCATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTa                                    <  1:326732/138‑1 (MQ=255)                                                                                                      tcCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGt                                                                       >  2:194320/1‑96 (MQ=255)                                                                                                      tcCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGt                                                                       <  1:194320/96‑1 (MQ=255)                                                                                                               cGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCAc                   <  1:189401/139‑1 (MQ=255)                                                                                                                 ttCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCg                 >  2:79165/1‑139 (MQ=255)                                                                                                                 ttCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCg                 >  2:434270/1‑139 (MQ=255)                                                                                                                 ttCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCg                 >  2:40032/1‑139 (MQ=255)                                                                                                                 ttCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCg                 >  2:347124/1‑139 (MQ=255)                                                                                                                 ttCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCg                 >  2:217060/1‑139 (MQ=255)                                                                                                                 ttCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCg                 >  2:199402/1‑139 (MQ=255)                                                                                                                 ttCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCg                 >  1:346158/1‑139 (MQ=255)                                                                                                                 ttCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCg                 >  1:410639/1‑139 (MQ=255)                                                                                                                    cGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCgggg              <  1:270729/139‑1 (MQ=255)                                                                                                                              ttATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCGGGGCGGTGACGc     <  2:289560/138‑1 (MQ=255)                                                                                                                                 ttGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCGGGGCGGTGACGCaga  <  2:402588/138‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       TATATATCTGGCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCGGGGCGGTGACGCAGA  >  NZ_CP009273/3882008‑3882273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑