Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,882,782 T→C F231F (TTT→TTC)  tnaA → tryptophanase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,882,7820TC93.9% 100.7 / ‑0.3 33F231F (TTT→TTC) tnaAtryptophanase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (20/11);  total (21/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.91e-01

CGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTTGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGATCACCCGCGAAACCTACAAATATGCCGATATGCTGGCGATGTCCGCCAAGAAAGATGCGATGGTGCC  >  NZ_CP009273/3882650‑3882913
                                                                                                                                    |                                                                                                                                   
cGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGaa                                                                                                                               <  2:416861/139‑1 (MQ=255)
     cGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGc                                                                                                                          <  1:290913/139‑1 (MQ=255)
         tatCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTa                                                                                                                       <  2:194297/138‑1 (MQ=255)
               tGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTtcat                                                                                                                 >  2:160238/1‑138 (MQ=255)
                 cAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTtcatc                                                                                                                >  1:256388/1‑137 (MQ=255)
                  aaCCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAag                                                                                                             >  1:163887/1‑139 (MQ=255)
                        cACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGt                                                                                                       >  2:137359/1‑139 (MQ=255)
                                aCTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAg                                                                                                <  1:4513/138‑1 (MQ=255)
                                  tctGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGaa                                                                                              >  2:382877/1‑138 (MQ=255)
                                    tGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATa                                                                                            >  1:70996/1‑138 (MQ=255)
                                     gCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATAc                                                                                           >  1:248613/1‑138 (MQ=255)
                                     gCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATAc                                                                                           >  1:310111/1‑138 (MQ=255)
                                            gtCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTg                                                                                   >  2:85818/1‑139 (MQ=255)
                                            gtCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTg                                                                                   <  2:163395/139‑1 (MQ=255)
                                                                           gATGTACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGATCACCCGCGAAACCTAc                                                    <  2:13270/139‑1 (MQ=255)
                                                                            aTGTACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGATCACCCGCGAAACCTAc                                                    >  1:210944/1‑138 (MQ=255)
                                                                               tACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGATCACCCGCGAAACCTACAAat                                                <  1:363717/139‑1 (MQ=255)
                                                                                    cATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGATCACCCGCGAAACCTACAAATATGcc                                           >  1:274026/1‑139 (MQ=255)
                                                                                       cgcgAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGATCACCCGCGAAACCTACAAATATGCCGa                                         >  1:125675/1‑138 (MQ=255)
                                                                                         cgAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTTGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACa                                                                                          <  1:261937/87‑1 (MQ=255)
                                                                                         cgAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTTGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACa                                                                                          >  2:261937/1‑87 (MQ=255)
                                                                                                   cGATATTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGATCACCCGCGAAACCTACAAATATGCCGATATGCTGGCGATg                            <  2:225155/139‑1 (MQ=255)
                                                                                                      tatTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGATCACCcgcg                                                            <  1:53352/104‑1 (MQ=255)
                                                                                                      tatTCCGGTGGTAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGATCACCcgcg                                                            >  2:53352/1‑104 (MQ=255)
                                                                                                                gtAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGATCACCCGCGAAACCTACAAata                                               <  2:33636/107‑1 (MQ=255)
                                                                                                                gtAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGATCACCCGCGAAACCTACAAata                                               >  1:33636/1‑107 (MQ=255)
                                                                                                                gtAATGGACTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGATCACCCGCGAAACCTACAAATATGCCGATATGCTGGCGATGTCCGCCaaga                  >  1:43900/1‑136 (MQ=255)
                                                                                                                        cTCCGCGCGCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGATCACCCGCGAAACCTACAAATATGCCGATATGCTGGCGATGTCCGCCAAGAAAGATGCGATg       >  2:24657/1‑139 (MQ=255)
                                                                                                                           cgcgcgCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGa                                                                     >  2:36560/1‑74 (MQ=255)
                                                                                                                           cgcgcgCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGa                                                                     <  1:36560/74‑1 (MQ=255)
                                                                                                                           cgcgcgCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGATCACCCGCGAAACCTACAAATATGCCGATATGCTGGCGATGTCCGCCAAGAAAGATGCGATGGTg    >  1:198892/1‑139 (MQ=255)
                                                                                                                           cgcgcgCTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGATCACCCGCGAAACCTACAAATATGCCGATATGCTGGCGATGTCCGCCAAGAAAGATGCGATGGTg    >  2:9795/1‑139 (MQ=255)
                                                                                                                             cgcgcTTCGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGATCACCCGCGAAACCTACAAATATGCCGATATGCTGGCGATGTCCGCCAAGAAAGATGCGATGGTGcc  >  1:88789/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                   
CGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGTACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCGCGCTTTGCTGAAAACGCCTATTTCATCAAGCAGCGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGATCACCCGCGAAACCTACAAATATGCCGATATGCTGGCGATGTCCGCCAAGAAAGATGCGATGGTGCC  >  NZ_CP009273/3882650‑3882913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑