| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,885,585 | T→C | F204S (TTC→TCC) | mdtL → | multidrug efflux MFS transporter MdtL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,885,585 | 0 | T | C | 98.1% | 180.0 / ‑3.8 | 52 | F204S (TTC→TCC) | mdtL | multidrug efflux MFS transporter MdtL |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (25/26); total (25/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01 | |||||||||||
TTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCT > NZ_CP009273/3885452‑3885718 | ttCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCt < 2:24958/137‑1 (MQ=255) cTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAg < 1:119719/138‑1 (MQ=255) cTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAg < 1:49627/138‑1 (MQ=255) gggCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAg > 2:203855/1‑136 (MQ=255) gggCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAg < 1:203855/136‑1 (MQ=255) gCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCgtg > 1:40245/1‑139 (MQ=255) gCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCgtg > 1:4922/1‑139 (MQ=255) gATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGtgtt > 1:225915/1‑139 (MQ=255) tGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCt > 1:224415/1‑127 (MQ=255) tGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCt < 2:224415/127‑1 (MQ=255) tGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGtgttgt > 2:422393/1‑139 (MQ=255) tGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGtgttgt > 1:330786/1‑139 (MQ=255) tGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGtgttgt > 1:258761/1‑139 (MQ=255) ggCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAg > 1:372618/1‑119 (MQ=255) ggCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAg < 2:372618/119‑1 (MQ=255) cgGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGt > 2:356907/1‑139 (MQ=255) aTGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGtgttgt > 2:265271/1‑113 (MQ=255) aTGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGtgttgt < 1:265271/113‑1 (MQ=255) gtCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATcac < 1:74167/114‑1 (MQ=255) gtCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATcac > 2:74167/1‑114 (MQ=255) gtCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCt > 2:294115/1‑138 (MQ=255) gtCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCt > 2:163672/1‑138 (MQ=255) gtCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTc > 2:29744/1‑139 (MQ=255) tGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACttt > 1:385869/1‑139 (MQ=255) tGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACttt > 1:119407/1‑139 (MQ=255) tGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACttt > 1:361467/1‑139 (MQ=255) ttttAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCaa < 2:225915/139‑1 (MQ=255) ttttAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCaa > 1:180425/1‑139 (MQ=255) ttttAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCaa > 2:162944/1‑139 (MQ=255) ttttAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCaa > 1:55405/1‑139 (MQ=255) aaaaGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACg > 1:128821/1‑139 (MQ=255) gAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCAc < 1:83076/139‑1 (MQ=255) gAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCAc < 1:358714/139‑1 (MQ=255) gAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCAc < 1:321230/139‑1 (MQ=255) gAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCAc < 1:371477/139‑1 (MQ=255) aCGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCgg > 2:174644/1‑139 (MQ=255) gcgcCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTa < 1:170577/139‑1 (MQ=255) gCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCa < 2:110623/139‑1 (MQ=255) cAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCAt < 1:356907/139‑1 (MQ=255) tAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCGCCACCCTCAGCGTTTCGGTGATCCTCACGTTCGTCAACACGTTACCGGTATTGCTGATGGAAATCATGGGGTTTGAg < 1:161914/139‑1 (MQ=255) tAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGGTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAg < 1:162944/139‑1 (MQ=255) aaCCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAgcg < 1:140593/139‑1 (MQ=255) aCCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAgcgc > 1:142265/1‑139 (MQ=255) ccACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGcgcg < 2:180425/139‑1 (MQ=255) ccACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGcgcg < 1:294115/139‑1 (MQ=255) gagaAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGa < 1:422393/139‑1 (MQ=255) agaAAATAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGaa < 2:40245/139‑1 (MQ=255) aaTAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACg > 1:326794/1‑139 (MQ=255) aTAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGc < 2:165778/139‑1 (MQ=255) aTAGCGAGTCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGc < 2:142265/139‑1 (MQ=255) tCGCTGCTTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTa < 2:249722/139‑1 (MQ=255) ctTAACCGTTTTTCCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGgcg < 1:115448/138‑1 (MQ=255) tttttcCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCt < 2:119407/139‑1 (MQ=255) tttcCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATa > 2:230108/1‑112 (MQ=255) tttcCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATa < 1:230108/112‑1 (MQ=255) | TTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCT > NZ_CP009273/3885452‑3885718 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |