Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,896,219 A→G T281T (ACT→ACC)  bglB ← 6‑phospho‑beta‑glucosidase BglB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,896,2190AG100.0% 131.7 / NA 38T281T (ACT→ACC) bglB6‑phospho‑beta‑glucosidase BglB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/16);  total (22/16)

CGGATTGGGGATCATATTCAGTATGTTGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCAGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGCCGGTTCTCTTCCATGGCCT  >  NZ_CP009273/3896084‑3896334
                                                                                                                                       |                                                                                                                   
cGGATTGGGGATCATATTCAGTATGTTGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGt                                                                                                                   <  1:48376/138‑1 (MQ=255)
      ggggATCATATTCAGTATGTTGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCAtctc                                                                                                             >  1:103341/1‑138 (MQ=255)
           tCATATTCAGTATGTTGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGt                                                                                                       <  2:289485/139‑1 (MQ=255)
                     tATGTTGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTgg                                                                                             >  2:273407/1‑139 (MQ=255)
                     tATGTTGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTgg                                                                                             >  2:344811/1‑139 (MQ=255)
                     tATGTTGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGAGATATTGTgg                                                                                             >  1:412388/1‑139 (MQ=255)
                      aTGTTGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGt                                                                                            >  2:360987/1‑139 (MQ=255)
                        gTTGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTcg                                                                                          >  1:254229/1‑139 (MQ=255)
                         ttGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTcgc                                                                                         <  1:190113/139‑1 (MQ=255)
                         ttGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTcgc                                                                                         >  1:206287/1‑139 (MQ=255)
                          tGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTcgcg                                                                                        >  1:175081/1‑139 (MQ=255)
                            cccTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGga                                                                                      <  1:125872/139‑1 (MQ=255)
                             ccTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCgg                                                                                       >  2:364770/1‑137 (MQ=255)
                               tGCGCATTTTTATTAATGCTTTCGTCGTGGGAAAAACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGAATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTAAATGGTGATATTGTGGTCGCGgaag                                                                                    >  1:131210/1‑138 (MQ=255)
                                 cgcATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGaaa                                                                                 >  1:155981/1‑139 (MQ=255)
                                 cgcATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGaaa                                                                                 >  2:105129/1‑139 (MQ=255)
                                   cATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACg                                                                               <  2:175081/139‑1 (MQ=255)
                                   cATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACg                                                                               <  1:67408/139‑1 (MQ=255)
                                      ttttATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACg                                                                               >  2:326159/1‑136 (MQ=255)
                                      ttttATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACg                                                                               <  1:326159/136‑1 (MQ=255)
                                                              aaaCACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCAcg                                                    <  1:364770/139‑1 (MQ=255)
                                                              aaaCACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCAcg                                                    <  2:103341/139‑1 (MQ=255)
                                                                 cacaACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACgcgc                                                 >  2:396640/1‑139 (MQ=255)
                                                                           cATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCa                                       >  2:122504/1‑139 (MQ=255)
                                                                               taataaCTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCa                                    <  1:290429/138‑1 (MQ=255)
                                                                                 ataaCTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCa                                    >  1:162837/1‑136 (MQ=255)
                                                                                  taaCTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAg                                 >  1:127076/1‑138 (MQ=255)
                                                                                    aCTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGaa                               >  1:311118/1‑138 (MQ=255)
                                                                                        aaaGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATc                           >  1:410629/1‑138 (MQ=255)
                                                                                        aaaGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATc                           >  2:96428/1‑138 (MQ=255)
                                                                                              aTGAATTCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCAAGCGCCTGAACATCACCAAAGAACATCCAGAGcc                    >  2:52494/1‑139 (MQ=255)
                                                                                                    tCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGCCGGTtct              <  2:162837/139‑1 (MQ=255)
                                                                                                    tCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGCCGGTtct              <  1:253121/139‑1 (MQ=255)
                                                                                                          gTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGCCGGTTCTCTTCCa        <  2:155981/139‑1 (MQ=255)
                                                                                                                 ttAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGCCGGTTCTCTTCCATGGCCt  <  2:254229/138‑1 (MQ=255)
                                                                                                                 ttAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGCCGGTTCTCTTCCATGGCCt  <  2:127076/138‑1 (MQ=255)
                                                                                                                         tctGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGa                                >  2:357945/1‑100 (MQ=255)
                                                                                                                         tctGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGa                                <  1:357945/100‑1 (MQ=255)
                                                                                                                                       |                                                                                                                   
CGGATTGGGGATCATATTCAGTATGTTGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCAGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGCCGGTTCTCTTCCATGGCCT  >  NZ_CP009273/3896084‑3896334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑