| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,963,488 | T→C | intergenic (+51/‑5) | wzzE → / → wecB | ECA polysaccharide chain length modulation protein/UDP‑N‑acetylglucosamine 2‑epimerase (non‑hydrolyzing) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,963,488 | 0 | T | C | 95.0% | 62.4 / ‑5.7 | 20 | intergenic (+51/‑5) | wzzE/wecB | ECA polysaccharide chain length modulation protein/UDP‑N‑acetylglucosamine 2‑epimerase (non‑hydrolyzing) |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (11/8); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCAT > NZ_CP009273/3963355‑3963609 | gtGCCTTCCTGATGATTATGTGGGTCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGAt < 1:326367/138‑1 (MQ=255) tGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGAtgt > 1:18379/1‑139 (MQ=255) ccTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGAtgtg > 1:22699/1‑138 (MQ=255) ggCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTgg > 2:354595/1‑138 (MQ=255) ggggCTGATCGGGGCGGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGa < 2:310708/139‑1 (MQ=255) gggCTGATCGGGGTGGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGCCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGaa < 2:370399/139‑1 (MQ=255) gATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCAt > 1:334092/1‑139 (MQ=255) gATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGGGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCAt > 1:13403/1‑139 (MQ=255) gtgtCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGaa > 2:421301/1‑123 (MQ=255) gtgtCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGaa < 1:421301/123‑1 (MQ=255) tCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGt < 2:202819/139‑1 (MQ=255) tCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGt < 1:354595/139‑1 (MQ=255) acacTGCTGCGGTGAGCGCAAAGGCGCTCTCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATTGCGCCGTTGGTGCATGCGTTGTCAAAAGATCCtttttt < 1:251590/139‑1 (MQ=255) ctgcGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGc > 1:209588/1‑139 (MQ=255) ctgcGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGc > 1:94623/1‑139 (MQ=255) ctgcGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGc > 1:309668/1‑139 (MQ=255) gTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAg > 2:97391/1‑139 (MQ=255) aGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGttt > 2:366745/1‑139 (MQ=255) cgcTCGCCGCTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTgc > 2:119317/1‑139 (MQ=255) cgcTTATTCGAAGAGAACCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCAt < 2:22699/139‑1 (MQ=255) | GTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCAT > NZ_CP009273/3963355‑3963609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |