Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,003,978	A→G	E140G (GAG→GGG)	yigL →	sugar/pyridoxal phosphate phosphatase YigL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,003,978	0	A	G	100.0%	70.1 / NA	21	E140G (GAG→GGG)	yigL	sugar/pyridoxal phosphate phosphatase YigL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/14);  total (7/14)

CAATCCGGACATCATTACTAACGTTTATCGCGACGACGAATGGTTTATGAATCGCCATCGCCCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGAGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGG  >  NZ_CP009273/4003850‑4004106                                                                                                                                 |                                                                                                                                 cAATCCGGACAGCATTGCTAACGTTTATCGCGACGACGAATGGTTTATGAATCGCCATCGCCCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGc                                                                                                                        <  1:121373/139‑1 (MQ=255)    tCCGGACATCATTACTAACGTTTATCGCGACGACGAATGGTTTATGAATCGCCATCGCCCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGt                                                                                                                      >  1:29683/1‑138 (MQ=255)          catcatTACTAACGTTTATCGCGACGACGAATGGTTTATGAATCGCCATCGCCCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCaaa                                                                                                               <  1:390538/139‑1 (MQ=255)                              gcgACGACGAATGGTTTATGAATCGCCATCGCCCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTc                                                                                           <  2:241258/139‑1 (MQ=255)                                       aaTGGTTTATGAATCGCCATCGCCCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGaaca                                                                                  <  2:263312/139‑1 (MQ=255)                                                      gccatcgccCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTg                                                                     >  1:360459/1‑137 (MQ=255)                                                          tcgccCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAg                                                               <  2:277296/139‑1 (MQ=255)                                                                                  tAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGa                                        <  2:112487/138‑1 (MQ=255)                                                                                       aagCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGt                                  <  1:319152/139‑1 (MQ=255)                                                                                                     tatGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTtct                    <  2:42951/139‑1 (MQ=255)                                                                                                     tatGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTtct                    <  1:141952/139‑1 (MQ=255)                                                                                                          gcTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTAcc               <  2:230949/139‑1 (MQ=255)                                                                                                                tGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTACCTTAAc          <  2:29683/138‑1 (MQ=255)                                                                                                                   gCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGa                                                                                              <  1:30173/51‑1 (MQ=255)                                                                                                                   gCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGa                                                                                              >  2:30173/1‑51 (MQ=255)                                                                                                                        gATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAAc                                                      <  2:242705/86‑1 (MQ=255)                                                                                                                        gATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAAc                                                      >  1:242705/1‑86 (MQ=255)                                                                                                                        gATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCa                          >  2:2594/1‑114 (MQ=255)                                                                                                                        gATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCa                          <  1:2594/114‑1 (MQ=255)                                                                                                                        gATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTgg  >  1:274596/1‑138 (MQ=255)                                                                                                                        gATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTgg  >  2:391411/1‑138 (MQ=255)                                                                                                                                 |                                                                                                                                 CAATCCGGACATCATTACTAACGTTTATCGCGACGACGAATGGTTTATGAATCGCCATCGCCCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGAGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGG  >  NZ_CP009273/4003850‑4004106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑