Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,052,334	A→G	I190V (ATT→GTT)	typA →	ribosome‑dependent GTPase TypA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,052,334	0	A	G	100.0%	72.7 / NA	23	I190V (ATT→GTT)	typA	ribosome‑dependent GTPase TypA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/10);  total (13/10)

TCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGATTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCGGTAAAGT  >  NZ_CP009273/4052204‑4052458                                                                                                                                   |                                                                                                                             tCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGAc                                                                                                                      >  2:256214/1‑139 (MQ=255)   tGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCa                                                                                                                    >  2:209635/1‑139 (MQ=255)          tAACCTCGACGCGACCGACGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCt                                                                                                             >  2:54042/1‑139 (MQ=255)                 gACGCGACCGACGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCCCCGTCCTGCGCCgg                                                                                                      >  2:237829/1‑139 (MQ=255)                              agcagcTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGa                                                                                         <  1:273269/139‑1 (MQ=255)                                                            cTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGCCATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGc                                                            <  2:200609/138‑1 (MQ=255)                                                            cTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGc                                                            <  1:435689/138‑1 (MQ=255)                                                                gcTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGAt                                                       <  1:423470/139‑1 (MQ=255)                                                                    aaCGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGAt                                                                          <  1:387053/116‑1 (MQ=255)                                                                    aaCGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGAt                                                                          >  2:387053/1‑116 (MQ=255)                                                                      cGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTacaac                                                 <  1:26488/139‑1 (MQ=255)                                                                              cgGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGAAGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTCCAACAGCTATGt                                         >  1:239214/1‑139 (MQ=255)                                                                                           cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGGCGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGc                            >  2:5009/1‑139 (MQ=255)                                                                                           cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCgg                             >  2:320379/1‑138 (MQ=255)                                                                                                          aGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAg             >  1:335010/1‑139 (MQ=255)                                                                                                          aGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAg             <  2:239214/139‑1 (MQ=255)                                                                                                           gACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAgc            >  1:144861/1‑139 (MQ=255)                                                                                                                 aCCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCGGt       <  2:335010/138‑1 (MQ=255)                                                                                                                   cccGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCgg        <  1:166709/135‑1 (MQ=255)                                                                                                                   cccGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCgg        >  2:166709/1‑135 (MQ=255)                                                                                                                     cGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTa                                                     >  2:422740/1‑88 (MQ=255)                                                                                                                     cGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTa                                                     <  1:422740/88‑1 (MQ=255)                                                                                                                     cGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCGGTAAAGt  >  2:329685/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                             TCTGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGATTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCGGTAAAGT  >  NZ_CP009273/4052204‑4052458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑