Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,064,260	A→C	V286G (GTG→GGG)	yihS ←	sulfoquinovose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,064,260	0	A	C	100.0%	123.8 / NA	35	V286G (GTG→GGG)	yihS	sulfoquinovose isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/15);  total (20/15)

ACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACGTACACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGCCTGGTGTACCTCCGAACG  >  NZ_CP009273/4064125‑4064395                                                                                                                                        |                                                                                                                                        aCGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACGTACACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCgg                                                                                                                                      <  2:41820/139‑1 (MQ=255)              cgTAGGCCGTACCCATTGCTTCGACGATAGGCCAACGTACACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTaaa                                                                                                                            >  2:409726/1‑136 (MQ=255)              cgTAGGCCGTACCCATTGCTTCGACGATAGGCCAACGTACACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTaaa                                                                                                                            >  1:200052/1‑136 (MQ=255)              cgTAGGCCGTACCCATTGCTTCGACGATAGGCCAACGTACACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTaaa                                                                                                                            >  1:22324/1‑136 (MQ=255)               gTAGGCCGTACCCATTGCTTCGACGATAGGCCAACGTACACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGa                                                                                                                        >  1:291737/1‑139 (MQ=255)                        aCCCATTGCTTCGACGATAGGCCAACGTACACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCa                                                                                                               >  1:285625/1‑139 (MQ=255)                         cccATTGCTTCGACGATAGGCCAACGTACACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCa                                                                                                               <  2:200052/138‑1 (MQ=255)                             ttGCTTCGACGATAGGCCAACGTACACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCAtcttc                                                                                                          >  2:142896/1‑139 (MQ=255)                             ttGCTTCGACGATAGGCCAACGTACACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCAtcttc                                                                                                          >  2:13159/1‑139 (MQ=255)                                 ttCGACGATAGGCCAACGTACACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTag                                                                                                       >  2:191198/1‑138 (MQ=255)                                   cgacgaTAGGCCAACGTACACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTagcag                                                                                                    >  2:212980/1‑139 (MQ=255)                                    gacgaTAGGCCAACGTACACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTagcagc                                                                                                   <  1:103259/139‑1 (MQ=255)                                    gacgaTAGGCCAACGTACACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTagcag                                                                                                    <  1:13159/138‑1 (MQ=255)                                       gaTAGGCCAACGTACACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCAt                                                                                                <  1:246029/139‑1 (MQ=255)                                                aCGTACACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTggtggt                                                                                       >  2:429414/1‑139 (MQ=255)                                                          tCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAAc                                                                             >  1:218485/1‑139 (MQ=255)                                                           cgcgGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACg                                                                            >  1:119155/1‑139 (MQ=255)                                                           cgcgGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACg                                                                            >  1:182825/1‑139 (MQ=255)                                                           cgcgGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACg                                                                            >  2:396656/1‑139 (MQ=255)                                                            gcgGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACg                                                                            <  1:191198/138‑1 (MQ=255)                                                                    ccGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAg                                                                   <  2:182825/139‑1 (MQ=255)                                                                      ggTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAggg                                                                 <  2:295383/139‑1 (MQ=255)                                                                      ggTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAggg                                                                 <  1:142896/139‑1 (MQ=255)                                                                                  cAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGCtgt                                                     >  2:297011/1‑139 (MQ=255)                                                                                       aaCGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTgcag                                                 <  2:285625/138‑1 (MQ=255)                                                                                            tataAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTaa                                           <  2:22324/139‑1 (MQ=255)                                                                                              taAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTaaa                                          <  1:396656/138‑1 (MQ=255)                                                                                                  cAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGcc                                     <  2:377329/139‑1 (MQ=255)                                                                                                     tCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCa                                  >  2:302415/1‑139 (MQ=255)                                                                                                     tCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCa                                  >  1:22440/1‑139 (MQ=255)                                                                                                       ccGTCCGCGCCATCGGGTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCAtt                                <  2:30618/139‑1 (MQ=255)                                                                                                                     gggTGCCCAGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGcc                   <  2:119155/138‑1 (MQ=255)                                                                                                                             aGGCATCGCGCCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGCCTGGTGTAcc          >  2:296190/1‑139 (MQ=255)                                                                                                                                    gcgcCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGCCTGGTGTACCTCCGAAc   >  2:110471/1‑139 (MQ=255)                                                                                                                                     cgcCCGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGCCTGGTGTACCTCCGAAcg  >  1:350145/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        ACGGTGTAGAGCGCGTAGGCCGTACCCATTGCTTCGACGATAGGCCAACGTACACGTTCGCGGACCACCGGTTTTCCTTCCCAGTCAACGGTATAAACAATCCCGTCCGCGCCATCGGGTGCCCAGGCATCGCGCACGGTGGCGTTAAACAGACCTTTGGCATCTTCTAGCAGCCATGCTGGTGGTTGTTCGCAACGGGCTTCCAGGGCAGCATGGATGTGCAGCATTAAACGGCCCCATTCGATCCAGTGGCCTGGTGTACCTCCGAACG  >  NZ_CP009273/4064125‑4064395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑