Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,132,297	T→C	intergenic (‑148/‑161)	ptsP ← / → frwC	phosphoenolpyruvate‑‑protein phosphotransferase/PTS fructose transporter subunit EIIC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,132,297	0	T	C	100.0%	85.0 / NA	24	intergenic (‑148/‑161)	ptsP/frwC	phosphoenolpyruvate‑‑protein phosphotransferase/PTS fructose transporter subunit EIIC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/13);  total (11/13)

GTACGGTTTTCTGTTTACAGGATAAGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACAT  >  NZ_CP009273/4132163‑4132430                                                                                                                                       |                                                                                                                                      gTACGGTTTTCTGTTTACAGGATAAGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCgg                                                                                                                                   >  2:59333/1‑139 (MQ=255) gTACGGTTTTCTGTTTACAGGATAAGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCgg                                                                                                                                   >  2:253624/1‑139 (MQ=255)  tACGGTTTTCTGTTTACAGGATAAGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGt                                                                                                                                  <  1:322348/139‑1 (MQ=255)                  cAGGATAAGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGa                                                                                                                  <  1:134865/139‑1 (MQ=255)                          gagCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATc                                                                                                          >  2:401026/1‑139 (MQ=255)                           agCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCg                                                                                                         <  1:260271/139‑1 (MQ=255)                               cACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCAtt                                                                                                     >  2:44645/1‑139 (MQ=255)                                    cAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGt                                                                                                <  1:17823/139‑1 (MQ=255)                                     aaCGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTa                                                                                               <  1:400151/139‑1 (MQ=255)                                                 tgtgACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACaa                                                                                   <  1:217296/139‑1 (MQ=255)                                                 tgtgACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACaa                                                                                   <  1:179296/139‑1 (MQ=255)                                                             gCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTaaa                                                                       >  1:392174/1‑139 (MQ=255)                                                                 aaaGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTG‑TTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGcc                                                                  <  2:100270/139‑1 (MQ=255)                                                                  aaGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGcc                                                                  <  1:287831/139‑1 (MQ=255)                                                                             atgtaatgtaACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCAc                                                       >  2:223155/1‑139 (MQ=255)                                                                                     taaCCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCt                                               <  2:227563/139‑1 (MQ=255)                                                                                       aCCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTcc                                             >  1:394210/1‑139 (MQ=255)                                                                                              aTTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCg                                      <  1:44645/139‑1 (MQ=255)                                                                                               tttGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGa                                     >  1:239651/1‑139 (MQ=255)                                                                                                              acaAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTtcc                      <  2:394210/139‑1 (MQ=255)                                                                                                                   aCGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCAt                 >  2:351218/1‑139 (MQ=255)                                                                                                                        gTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCaaa             >  1:153507/1‑138 (MQ=255)                                                                                                                                 cATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACa   >  2:347925/1‑139 (MQ=255)                                                                                                                                  aTGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACAt  <  1:223155/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      GTACGGTTTTCTGTTTACAGGATAAGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACAT  >  NZ_CP009273/4132163‑4132430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑