Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,223,121	A→G	V14A (GTA→GCA)	lysC ←	lysine‑sensitive aspartokinase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,223,121	0	A	G	100.0%	124.4 / NA	33	V14A (GTA→GCA)	lysC	lysine‑sensitive aspartokinase 3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/16);  total (17/16)

CTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTATTT  >  NZ_CP009273/4223002‑4223252                                                                                                                        |                                                                                                                                     cTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAAttt                                                                                                                   >  2:137516/1‑139 (MQ=255)    cAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCTCCAAATTTg                                                                                                                  >  2:116121/1‑137 (MQ=255)    cAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTg                                                                                                                  <  2:357554/137‑1 (MQ=255)    cAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTg                                                                                                                  <  2:335718/137‑1 (MQ=255)      gCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGgaga                                                                                                              >  2:374247/1‑139 (MQ=255)       cTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGac                                                                                                             <  2:374206/139‑1 (MQ=255)                   gcagATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGa                                                                                                 <  2:172358/139‑1 (MQ=255)                              aTACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTAcc                                                                                       <  1:440767/138‑1 (MQ=255)                                 ccAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCgt                                                                                   >  1:23463/1‑139 (MQ=255)                                     cagAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCgtgt                                                                                 >  2:260203/1‑137 (MQ=255)                                     cagAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCgtgt                                                                                 >  1:402022/1‑137 (MQ=255)                                        aaGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑ggg                                                                           <  2:23463/139‑1 (MQ=255)                                          gCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGa                                                                         >  2:427711/1‑139 (MQ=255)                                            cGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATc                                                                       <  2:300805/139‑1 (MQ=255)                                            cGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATc                                                                       >  2:72098/1‑139 (MQ=255)                                                          aaCGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATCCATTTTCAGCCt                                                           <  1:308403/137‑1 (MQ=255)                                                            cGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATCCATTTTCAGCCTTgg                                                        >  2:328244/1‑138 (MQ=255)                                                                    ggCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATCCATTTTCAGCCTTGGCACAAggg                                                <  1:278117/138‑1 (MQ=255)                                                                        tCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATCCATTTTCAGCCTTGGCACAAGGGAagag                                           <  1:10849/139‑1 (MQ=255)                                                                               gCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGa                                    <  1:272123/139‑1 (MQ=255)                                                                                          gcgcTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGgcg                          >  2:208527/1‑138 (MQ=255)                                                                                          gcgcTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGgcg                          >  1:281145/1‑138 (MQ=255)                                                                                           cgcTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGgcg                          >  1:141722/1‑137 (MQ=255)                                                                                             cTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACggg                                <  1:3428/129‑1 (MQ=255)                                                                                             cTGCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACggg                                >  2:3428/1‑129 (MQ=255)                                                                                               gCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATCCATTTTCAGCCt                                                           >  2:24005/1‑100 (MQ=255)                                                                                               gCGGTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATCCATTTTCAGCCt                                                           <  1:24005/100‑1 (MQ=255)                                                                                                 ggTTCATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGa                  <  2:281145/139‑1 (MQ=255)                                                                                                     cATGGCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATAc               >  2:118163/1‑139 (MQ=255)                                                                                                        ggCGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCg           >  2:7537/1‑139 (MQ=255)                                                                                                          cGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATCCATTTTCAGCCTTGGCACAAGGGAagag                                           >  1:346054/1‑105 (MQ=255)                                                                                                          cGTCAAAATCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATCCATTTTCAGCCTTGGCACAAGGGAagag                                           <  2:346054/105‑1 (MQ=255)                                                                                                                 aTCAGCTGCGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTAttt  <  1:100887/139‑1 (MQ=255)                                                                                                                        |                                                                                                                                     CTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCA‑GGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTATTT  >  NZ_CP009273/4223002‑4223252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑