Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,262,930	A→T	D290E (GAT→GAA)	uvrA ←	excinuclease ABC subunit UvrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,262,930	0	A	T	100.0%	62.6 / NA	17	D290E (GAT→GAA)	uvrA	excinuclease ABC subunit UvrA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (9/8);  total (9/8)

CTTCGACGTCGAACTTATAGTGATCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGCCCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAGAACAG  >  NZ_CP009273/4262800‑4263064                                                                                                                                   |                                                                                                                                       cTTCGACGTCGAACTTATAGTGATCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAata                                                                                                                                >  2:169158/1‑139 (MQ=255)       cGTCGAACTTATAGTGATCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTg                                                                                                                          <  1:307990/139‑1 (MQ=255)         tCGAACTTATAGTGATCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTa                                                                                                                        >  2:230999/1‑139 (MQ=255)                                             tGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCgg                                                                                   >  1:134897/1‑139 (MQ=255)                                                  ataataGAATTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGgttgt                                                                              <  1:51264/139‑1 (MQ=255)                                                         aaGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGACGGGCAGG‑CCCCGCCGGGTTGTTAAACg                                                                        >  2:72527/1‑138 (MQ=255)                                                                               ggATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGccccgcc                                                                                      >  1:425381/1‑100 (MQ=255)                                                                               ggATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGccccgcc                                                                                      <  2:425381/103‑4 (MQ=255)                                                                                gATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCAc                                                <  1:342494/139‑1 (MQ=255)                                                                                    gCACCACAAGCCAGCGACAGTTCCGGATGCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGACAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCa                                             <  1:425014/138‑1 (MQ=255)                                                                                                agcGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTg                                        >  1:236880/1‑131 (MQ=255)                                                                                                agcGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTg                                        <  2:236880/131‑1 (MQ=255)                                                                                                                  tGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCa                                             <  2:147168/108‑1 (MQ=255)                                                                                                                  tGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCa                                             >  1:147168/1‑108 (MQ=255)                                                                                                                         cTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCggc        >  1:111394/1‑137 (MQ=255)                                                                                                                         cTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCg          >  2:233927/1‑136 (MQ=255)                                                                                                                              tCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAGAAcag  <  2:134897/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       CTTCGACGTCGAACTTATAGTGATCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGCCCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAGAACAG  >  NZ_CP009273/4262800‑4263064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑