| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,321,803 | T→C | H495H (CAT→CAC) | proP → | glycine betaine/L‑proline transporter ProP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,321,803 | 0 | T | C | 96.4% | 85.3 / ‑3.3 | 28 | H495H (CAT→CAC) | proP | glycine betaine/L‑proline transporter ProP |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (10/17); total (11/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.45e-01 | |||||||||||
AGGAAGCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTG > NZ_CP009273/4321688‑4321930 | aGGAAGCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTg > 2:81401/1‑138 (MQ=255) gCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACgg < 2:40758/139‑1 (MQ=255) gCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACg > 2:249597/1‑138 (MQ=255) gtcgAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGAc < 1:249597/139‑1 (MQ=255) gtcgAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGAc < 2:150556/139‑1 (MQ=255) tcgAGCATTACGATAATATCGAGCAGAAAACCGATGATATTGACCACGAGGTTGCGGATTTGCAGGCGAAAGGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACg < 2:326316/139‑1 (MQ=255) ttACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCt > 1:329746/1‑138 (MQ=255) atatCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGc < 2:329746/138‑1 (MQ=255) tatCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACg > 1:204084/1‑123 (MQ=255) tatCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACg < 2:204084/123‑1 (MQ=255) gAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTg < 1:81401/139‑1 (MQ=255) cgCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTg > 1:157636/2‑138 (MQ=255) aGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTg > 1:431374/1‑138 (MQ=255) gCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGaa < 2:327547/139‑1 (MQ=255) aaTCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCtt > 2:383841/1‑138 (MQ=255) atTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACt > 1:142848/1‑138 (MQ=255) cGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGGGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATctct > 2:249626/1‑138 (MQ=255) gagaTTGCCGATTTGCAGGCGAACCGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGACCTCTTAAGGTTCACTTAATCTCTGa < 1:249626/139‑1 (MQ=255) gaTTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGAcg < 2:282898/139‑1 (MQ=255) aTTGCCGATGTGCAGCCGAAACGTGCCCGCCTGGTGCAGCATCACCCGCGAATTGATGAATAATGTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAAGTGAACCCTTAAGGTTCACTTAAGCTCTTAcgc < 2:232245/139‑1 (MQ=255) gAAACGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAgg > 2:417857/1‑103 (MQ=255) gAAACGTACCAGCCTGGTGCAGCAACACCCTCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAgg < 1:417857/103‑1 (MQ=255) aaaCGTACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGt < 2:125151/139‑1 (MQ=255) tACCCGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACg < 2:7688/139‑1 (MQ=255) cccGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACg > 2:211102/1‑137 (MQ=255) cGCCTGGTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACggagg < 1:211102/139‑1 (MQ=255) gTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTg < 2:142848/139‑1 (MQ=255) gTGCAGCAACACCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTg < 2:74075/139‑1 (MQ=255) | AGGAAGCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTG > NZ_CP009273/4321688‑4321930 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |