Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,374,268	T→C	S205P (TCC→CCC)	yjeM →	glutamate/gamma‑aminobutyrate family transporter YjeM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,374,268	0	T	C	100.0%	102.6 / NA	29	S205P (TCC→CCC)	yjeM	glutamate/gamma‑aminobutyrate family transporter YjeM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/12);  total (17/12)

CTGCGGTGGGCGGTATTGCAGTAATGTGTCTGAATTTAGTATTGCTGTTAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGTCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGCCA  >  NZ_CP009273/4374134‑4374379                                                                                                                                       |                                                                                                                cTGCGGTGGGCGGTATTGCAGTAATGTGTCTGAATTTAGTATTGCTGTTAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCgg                                                                                                             <  2:111821/139‑1 (MQ=255)      gTGGGCGGTATTGCAGTAATGTGTCTGAATTTAGTATTGCTGTTAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTgg                                                                                                        >  1:111859/1‑139 (MQ=255)                ttGCAGTAATGTGTCTGAATTTAGTATTGCTGTTAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATc                                                                                              <  1:332657/139‑1 (MQ=255)                        aTGTGTCTGAATTTAGTATTGCTGTTAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTgg                                                                                      >  2:405738/1‑139 (MQ=255)                             tCTGAATTTAGTATTGCTGTTAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTAttt                                                                                 >  2:390601/1‑139 (MQ=255)                                                  aGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATgg                                                                <  1:219753/135‑1 (MQ=255)                                                  aGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATgg                                                                >  2:219753/1‑135 (MQ=255)                                                  aGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCgg                                                             >  2:64150/1‑138 (MQ=255)                                                  aGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCgg                                                             >  2:323000/1‑138 (MQ=255)                                                        cATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGaa                                                      <  1:64150/139‑1 (MQ=255)                                                          ttACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGc                                                    <  2:266570/139‑1 (MQ=255)                                                            aCTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATgg                                                                <  1:55204/125‑1 (MQ=255)                                                            aCTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATgg                                                                >  2:55204/1‑125 (MQ=255)                                                             cTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGt                                                 <  1:390601/139‑1 (MQ=255)                                                                ttttGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATgg                                                                >  1:410874/1‑121 (MQ=255)                                                                ttttGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATgg                                                                <  2:272604/121‑1 (MQ=255)                                                                ttttGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATgg                                                                >  1:272604/1‑121 (MQ=255)                                                                ttttGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATgg                                                                <  2:410874/121‑1 (MQ=255)                                                                   tGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTtggtgg                                           >  1:116731/1‑139 (MQ=255)                                                                     ttattaAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGtggtc                                         >  1:198080/1‑139 (MQ=255)                                                                       attaAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGtggtctg                                       >  1:141867/1‑139 (MQ=255)                                                                                tggGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTtggtg                                            <  1:435255/125‑1 (MQ=255)                                                                                tggGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTtggtg                                            >  2:435255/1‑125 (MQ=255)                                                                                  ggCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAAc                            >  1:311389/1‑139 (MQ=255)                                                                                         cgcgCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGtggtctg                                       >  1:310700/1‑121 (MQ=255)                                                                                         cgcgCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGtggtctg                                       <  2:310700/121‑1 (MQ=255)                                                                                                          tCCTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGc    >  1:114400/1‑139 (MQ=255)                                                                                                            cTTGCATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGcc   <  2:116731/138‑1 (MQ=255)                                                                                                              ttcATCACCGAACCCGGGTTATCAGCCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGCCa  >  1:36689/3‑137 (MQ=255)                                                                                                                                       |                                                                                                                CTGCGGTGGGCGGTATTGCAGTAATGTGTCTGAATTTAGTATTGCTGTTAGTAAGCATTACTATTTTGTTATTAAATGGTGGGCATTTCGCGCAGGATATTAATTTCCTTGCATCACCGAACCCGGGTTATCAGTCCGGTCTGGCAATGCTATCGTTTGTGGTATTTGCCATTTTTGCCTATGGCGGAATTGAAGCGGTTGGTGGTCTGGTCGATAAAACGGAAAATCCAGAAAAGAACTTTGCCA  >  NZ_CP009273/4374134‑4374379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑