Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 4,377,323 T→C D622G (GAC→GGC)  mscM ← miniconductance mechanosensitive channel MscM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,377,3230TC100.0% 80.3 / NA 24D622G (GAC→GGC) mscMminiconductance mechanosensitive channel MscM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/12);  total (12/12)

GTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGTCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCATCCCACGGGAAACACGTTCGCGCGGCCAGCCAAAATGAGCGATAAACAAGCCGTTCGGG  >  NZ_CP009273/4377188‑4377457
                                                                                                                                       |                                                                                                                                      
gtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGgccgt                                                                                                                                     >  2:216701/1‑139 (MQ=255)
gtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGgccgt                                                                                                                                     >  1:305778/1‑139 (MQ=255)
       tgtCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGt                                                                                                                               >  2:286148/1‑138 (MQ=255)
         tCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTAt                                                                                                                            >  2:301003/1‑139 (MQ=255)
                   ccactTTGTTGAGATACAGCGGAACCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATGCACGGCCGTCGAGGTTATCGAAcatcat                                                                                                                  <  2:243889/136‑1 (MQ=255)
                       tttGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACAtcatca                                                                                                                <  1:125948/137‑1 (MQ=255)
                           ttgAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGcc                                                                                                           <  1:286148/138‑1 (MQ=255)
                                           tCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAAt                                                                                          <  2:358800/139‑1 (MQ=255)
                                              cGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAg                                                                                       >  1:152301/1‑139 (MQ=255)
                                                        cAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCt                                                                              <  2:96528/138‑1 (MQ=255)
                                                         aGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCtca                                                                            <  1:216701/139‑1 (MQ=255)
                                                            cTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCtcatca                                                                         <  1:301003/139‑1 (MQ=255)
                                                                     accaccGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCtca                                                                            <  2:22101/127‑1 (MQ=255)
                                                                     accaccGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCtca                                                                            >  1:22101/1‑127 (MQ=255)
                                                                     accaccGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAgc                                                                >  1:97141/1‑139 (MQ=255)
                                                                         ccGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCAt                                                            >  1:230166/1‑139 (MQ=255)
                                                                                   aCCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCATCCCACGGGa                                                   >  2:26667/1‑138 (MQ=255)
                                                                                               gATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCATCCCACGGGAAACACGTTcgcgc                                      >  1:401368/1‑139 (MQ=255)
                                                                                                              tCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGAt                                                                                 >  1:81989/1‑81 (MQ=255)
                                                                                                              tCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGAt                                                                                 <  2:81989/81‑1 (MQ=255)
                                                                                                              tCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCATCCCACGGGAAACACGTTCGCGCGGCCAGCCAAAAt                         >  1:6645/1‑137 (MQ=255)
                                                                                                                ccAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCATCCCACGGGAAACACGTTCGCGCGGCCAGCCAAAATGAGc                     <  1:156898/139‑1 (MQ=255)
                                                                                                                                   aCGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGgta                                                                     <  2:155566/72‑1 (MQ=255)
                                                                                                                                   aCGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGgta                                                                     >  1:155566/1‑72 (MQ=255)
                                                                                                                                   aCGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCATCCCACGGGAAACACGTTCGCGCGGCCAGCCAAAATGAGCGATAAACAAGCCGTTcggg  >  1:432882/1‑139 (MQ=255)
                                                                                                                                   aCGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCATCCCACGGGAAACACGTTCGCGCGGCCAGCCAAAATGAGCGATAAACAAGCCGTTcggg  <  2:230166/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
GTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGTCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCATCCCACGGGAAACACGTTCGCGCGGCCAGCCAAAATGAGCGATAAACAAGCCGTTCGGG  >  NZ_CP009273/4377188‑4377457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑