Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,378,349	T→C	D280G (GAT→GGT)	mscM ←	miniconductance mechanosensitive channel MscM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,378,349	0	T	C	100.0%	67.1 / NA	19	D280G (GAT→GGT)	mscM	miniconductance mechanosensitive channel MscM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/6);  total (13/6)

CGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTC  >  NZ_CP009273/4378239‑4378476                                                                                                               |                                                                                                                                cGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGACCCATCCGCTGCGCCTGTTGATTCAAAgc                                                                                                     <  2:304525/139‑1 (MQ=255)        aTTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGACCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGa                                                                                               >  2:341411/1‑138 (MQ=255)                  cccAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGACCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGt                                                                                    >  2:20173/1‑139 (MQ=255)                  cccAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGACCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGt                                                                                    >  2:102023/1‑139 (MQ=255)                           tGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGCGGCAACGAGACCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTg                                                                            >  2:436058/1‑138 (MQ=255)                           tGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGACCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTg                                                                            >  1:177098/1‑138 (MQ=255)                           tGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGACCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTg                                                                            >  1:398949/1‑138 (MQ=255)                                  gTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGACCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTgcgc                                                                    >  2:303793/1‑139 (MQ=255)                                     cACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGACCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGcgac                                                                 >  2:346782/1‑139 (MQ=255)                                            cGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGACCCATCCGCTGCGCCTGTTGATTCAAAgc                                                                                                     <  2:222856/96‑1 (MQ=255)                                            cGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGACCCATCCGCTGCGCCTGTTGATTCAAAgc                                                                                                     >  1:222856/1‑96 (MQ=255)                                             gTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGACCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCtt                                                         >  1:352785/1‑139 (MQ=255)                                             gTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGACCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCtt                                                         >  2:247176/1‑139 (MQ=255)                                                      gCCTGCCGGACCTGTAACGTCTGGCTGGTAGCCTGACGCTGTTGCGAGGCAACGAGACCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAAt                                                >  2:166626/1‑139 (MQ=255)                                                                         tCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGACCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAg                             <  1:247176/139‑1 (MQ=255)                                                                              ctggcAGCCTTACGCTGTTGCGAGGCAACGAGACCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAAtt                        <  1:16785/139‑1 (MQ=255)                                                                              ctggcAGCCTGACGCTGTTGCGAGGCAACGAGACCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAAtt                        <  2:177098/139‑1 (MQ=255)                                                                                   aGCCTGACGCTGTTGCGAGGCAACGAGACCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCGGTa                   <  2:352785/139‑1 (MQ=255)                                                                                                    aGGCAACGAGACCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTc  >  2:196439/1‑139 (MQ=255)                                                                                                               |                                                                                                                                CGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTC  >  NZ_CP009273/4378239‑4378476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑