Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,395,911	T→C	intergenic (+109/‑96)	purA → / → nsrR	adenylosuccinate synthase/nitric oxide‑sensing transcriptional repressor NsrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,395,911	0	T	C	100.0%	128.2 / NA	36	intergenic (+109/‑96)	purA/nsrR	adenylosuccinate synthase/nitric oxide‑sensing transcriptional repressor NsrR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/13);  total (23/13)

CGCGACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCG  >  NZ_CP009273/4395782‑4396045                                                                                                                                  |                                                                                                                                       cgcgACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTcccc                                                                                                                                >  2:320946/1‑138 (MQ=255) cgcgACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTcccc                                                                                                                                >  2:102920/1‑138 (MQ=255)      cccGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACt                                                                                                                           >  2:153881/1‑138 (MQ=255)      cccGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACt                                                                                                                           >  2:335769/1‑138 (MQ=255)          ttCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGaaat                                                                                                                      <  2:288759/139‑1 (MQ=255)             gACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGaaataa                                                                                                                    <  2:224400/138‑1 (MQ=255)                      ttCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGaca                                                                                                          <  1:335769/139‑1 (MQ=255)                      ttCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGaca                                                                                                          <  2:407900/139‑1 (MQ=255)                                ccTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTgg                                                                                                <  2:57604/139‑1 (MQ=255)                                ccTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTgg                                                                                                >  1:258760/1‑139 (MQ=255)                                             ttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTatcat                                                                                    <  2:258760/138‑1 (MQ=255)                                                cctgccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCaa                                                                                 <  2:332211/138‑1 (MQ=255)                                                              aGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGATTGTGGCTGGTTTATAATCAATATAAATGTAttttt                                                                  >  2:65592/1‑139 (MQ=255)                                                                 gtgATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTcc                                                               >  2:429483/1‑139 (MQ=255)                                                                  tgATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAata                                                                               <  1:73011/122‑1 (MQ=255)                                                                  tgATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAata                                                                               >  2:73011/1‑122 (MQ=255)                                                                          tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTccc                                                      >  1:43618/1‑139 (MQ=255)                                                                          tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTccc                                                      >  2:243625/1‑139 (MQ=255)                                                                          tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTccc                                                      >  2:258022/1‑139 (MQ=255)                                                                          tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTccc                                                      >  1:13824/1‑139 (MQ=255)                                                                            cTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCtt                                                    >  1:244442/1‑139 (MQ=255)                                                                               tgtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTatcct                                                                                    >  2:358077/1‑102 (MQ=255)                                                                               tgtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTatcat                                                                                    <  1:358077/104‑1 (MQ=255)                                                                               tgtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTg                                                 >  1:372480/1‑139 (MQ=255)                                                                                        aGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtg                                        <  1:139218/139‑1 (MQ=255)                                                                                          ccATTACGCTTACCAACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTAGTTTATCATCAATATAAATGTATTTTTTCCCGATTACCCTTTTGAGGTTGAtgtg                                      <  1:189104/139‑1 (MQ=255)                                                                                          ccATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgtg                                      <  2:13824/139‑1 (MQ=255)                                                                                                          cacaGTGTTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCa                      >  2:209537/1‑139 (MQ=255)                                                                                                               tgtTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGaca                                                                                                          <  1:73797/50‑1 (MQ=255)                                                                                                               tgtTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGaca                                                                                                          >  2:73797/1‑50 (MQ=255)                                                                                                               tgtTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgat                 >  2:165022/1‑139 (MQ=255)                                                                                                               tgtTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgat                 >  1:919/1‑139 (MQ=255)                                                                                                               tgtTAAATCCTCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgat                 >  1:364750/1‑139 (MQ=255)                                                                                                                         tCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattac       >  2:15147/1‑139 (MQ=255)                                                                                                                         tCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattac       >  1:253468/1‑139 (MQ=255)                                                                                                                         tCGCTTTTTCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattac       >  1:179760/1‑139 (MQ=255)                                                                                                                               tttcCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgcg  <  2:372480/138‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       CGCGACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCG  >  NZ_CP009273/4395782‑4396045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑