Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,409,650	T→C	intergenic (‑208/‑147)	ulaG ← / → ulaA	L‑ascorbate 6‑phosphate lactonase/PTS ascorbate transporter subunit IIC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,409,650	0	T	C	100.0%	54.0 / NA	17	intergenic (‑208/‑147)	ulaG/ulaA	L‑ascorbate 6‑phosphate lactonase/PTS ascorbate transporter subunit IIC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/9);  total (8/9)

CAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAGGGAAGTTGTTGTTGATTTTGAGTATGGAAAGATTTAATGGAATGTGTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAATCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAATTAGAGTGAGTGCACAACATTCCGGGTGTGTGGAATACCCGGTTAC  >  NZ_CP009273/4409517‑4409776                                                                                                                                      |                                                                                                                               cAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAGGGAAGTTGTTGTTGATTTTGAGTATGGAAAGATTTAATGGAATGTGTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCa                                                                                                                            <  2:118717/138‑1 (MQ=255)                  ttttttGACTTTTTGCCAGGGAAGTTGTTGTTGATTTTGAGTATGGAAAGATTTAATGGAATGTGTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAat                                                                                                           >  1:364879/1‑138 (MQ=255)                             tttGCCAGGGAAGTTGTTGTTGATTTTGAGTATGGAAAGATTTAATGGAATGTGTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTc                                                                                               >  2:2867/1‑139 (MQ=255)                             tttGCCAGGGAAGTTGTTGTTGATTTTGAGTATGGAAAGATTTAATGGAATGTGTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTc                                                                                               >  2:271917/1‑139 (MQ=255)                                                  gATTTTGAGTATGGAAAGATTTAATGGAATGTGTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCataaa                                                                           <  2:364879/138‑1 (MQ=255)                                                             tGGAAAGATTTAATGGAATGTGTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTtgttgt                                                               >  1:422418/1‑139 (MQ=255)                                                                aaaGATTTAATGGAATGTGTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGAt                                                            <  2:61167/139‑1 (MQ=255)                                                                                 tgtAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAATTag                                            <  2:37792/138‑1 (MQ=255)                                                                                   tAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAATTAgagt                                         <  1:2867/139‑1 (MQ=255)                                                                                   tAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAATTAgagt                                         <  1:271917/139‑1 (MQ=255)                                                                                      ttCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAATTAgagtgag                                      >  1:288662/1‑139 (MQ=255)                                                                                           ttAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAATTAGAGTGAGTGcac                                 <  2:149725/139‑1 (MQ=255)                                                                                                                  tAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAATTag                                            <  1:287054/105‑1 (MQ=255)                                                                                                                  tAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAATTag                                            >  2:287054/1‑105 (MQ=255)                                                                                                                          tCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAATTAGAGTGAGTGCACAACATTCCGGGTGTGTGGAATAc          >  1:49236/1‑131 (MQ=255)                                                                                                                          tCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAATTAGAGTGAGTGCACAACATTCCGGGTGTGTGGAATAc          <  2:49236/131‑1 (MQ=255)                                                                                                                          tCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAATTAGAGTGAGTGCACAACATTCCGGGTGTGTGGAATACCCGGTTAc  >  1:194033/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                               CAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAGGGAAGTTGTTGTTGATTTTGAGTATGGAAAGATTTAATGGAATGTGTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAATCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAATTAGAGTGAGTGCACAACATTCCGGGTGTGTGGAATACCCGGTTAC  >  NZ_CP009273/4409517‑4409776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑