Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,428,964	T→C	E39G (GAG→GGG)	ytfJ ←	YtfJ family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,428,964	0	T	C	100.0%	78.9 / NA	23	E39G (GAG→GGG)	ytfJ	YtfJ family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/15);  total (8/15)

CAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCCTTCTTATGATA  >  NZ_CP009273/4428836‑4429099                                                                                                                                 |                                                                                                                                        cAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGa                                                                                                                                 <  1:270985/137‑1 (MQ=255)                              gagGTGCGACCAGCAATATGTTGCAGTGCTCGCACTTTTCCCACTAACGGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGAc                                                                                                  <  1:178874/139‑1 (MQ=255)                              gagGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGAc                                                                                                  <  1:257455/139‑1 (MQ=255)                                    cGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGttt                                                                                            >  1:417313/1‑139 (MQ=255)                                           cAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACt                                                                                      >  2:331859/1‑138 (MQ=255)                                                  ttGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCg                                                                              <  1:331859/139‑1 (MQ=255)                                                                  tttCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGc                                                               >  1:398261/1‑138 (MQ=255)                                                                   ttCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCaa                                                             >  1:289156/1‑139 (MQ=255)                                                                            aCTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCCCCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAgcagg                                                      <  1:87315/137‑1 (MQ=255)                                                                             cTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAgcaggcag                                                   >  1:286497/1‑139 (MQ=255)                                                                                cgcgcTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTg                                                <  2:242599/139‑1 (MQ=255)                                                                                  cgcTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGAt                                                                                   <  1:22676/102‑1 (MQ=255)                                                                                  cgcTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGAt                                                                                   >  2:22676/1‑102 (MQ=255)                                                                                            ggTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATc                                    <  1:274467/139‑1 (MQ=255)                                                                                                   tAGCTAAACTGATCTTTATCAAGCACCACCCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTa                             <  1:167049/139‑1 (MQ=255)                                                                                                   tAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTa                             <  1:348238/139‑1 (MQ=255)                                                                                                                   tATCAAGCACCAACCCGCCCCGATCGGTAATGCCAAGCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTc              <  1:43409/138‑1 (MQ=255)                                                                                                                    aTCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTcc             <  2:417313/138‑1 (MQ=255)                                                                                                                        aGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCcttct         >  1:184595/1‑138 (MQ=255)                                                                                                                         gCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCCTTCTTa       <  2:73961/139‑1 (MQ=255)                                                                                                                             cAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCCTTCTTATGat   >  2:217279/1‑139 (MQ=255)                                                                                                                              aaCCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCCTTCTTATGata  <  2:159646/139‑1 (MQ=255)                                                                                                                              aaCCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCCTTCTTATGata  <  1:244668/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                        CAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCCTTCTTATGATA  >  NZ_CP009273/4428836‑4429099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑