Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,501,175	T→C	I34V (ATC→GTC)	fecE ←	Fe(3+) dicitrate ABC transporter ATP‑binding protein FecE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,501,175	0	T	C	100.0%	76.4 / NA	22	I34V (ATC→GTC)	fecE	Fe(3+) dicitrate ABC transporter ATP‑binding protein FecE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/12);  total (10/12)

CCAACTGGCGCGATGAGAGCATATTTATGGGATTATCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGATCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCA  >  NZ_CP009273/4501052‑4501294                                                                                                                            |                                                                                                                        ccAACTGGCGCGATGAGAGCATATTTATGGGATTATCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCtt                                                                                                          <  2:292867/139‑1 (MQ=255)    aCTGGCGCGATGAGAGCATATTTATGGGATTATCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTccc                                                                                                       <  2:435064/139‑1 (MQ=255)        gcgcGATGAGAGCATATTTATGGGATTATCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCg                                                                                                     <  1:178567/137‑1 (MQ=255)             aTGAGAGCATATTTATGGGATTATCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTGCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAg                                                                                              <  2:112574/139‑1 (MQ=255)                agagCATATTTATGGGATTATCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTga                                                                                           <  1:23822/139‑1 (MQ=255)                           aTGGGATTATCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAAcg                                                                                 <  2:4742/138‑1 (MQ=255)                              ggATTATCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAAcgtcgt                                                                             >  2:167073/1‑139 (MQ=255)                                 ttATCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTaa                                                                          <  2:145039/139‑1 (MQ=255)                                   aTCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAg                                                                         >  2:55267/1‑138 (MQ=255)                                   aTCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAg                                                                         >  1:440039/1‑138 (MQ=255)                                    tCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAATGTCGTTAAGt                                                                        <  2:398964/138‑1 (MQ=255)                                                      ccAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAAc                                                     >  1:421937/1‑139 (MQ=255)                                                              cGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAg                                                                         >  2:382977/1‑111 (MQ=255)                                                              cGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGt                                              >  2:271825/1‑138 (MQ=255)                                                              cGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGt                                              >  1:250460/1‑138 (MQ=255)                                                              cGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTCAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAg                                                                         <  1:382977/111‑1 (MQ=255)                                                                           cgcgAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTc                                >  2:174648/1‑139 (MQ=255)                                                                                aaaaCAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTaaa                           <  2:421937/139‑1 (MQ=255)                                                                                 aaaCAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAg                          <  1:114228/139‑1 (MQ=255)                                                                                               cGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAACGTCATTTATCGCAtt            >  2:38135/1‑139 (MQ=255)                                                                                                 tCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTCGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATtct          >  1:321061/1‑139 (MQ=255)                                                                                                         ccGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCa  <  1:167073/139‑1 (MQ=255)                                                                                                                            |                                                                                                                        CCAACTGGCGCGATGAGAGCATATTTATGGGATTATCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGATCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCA  >  NZ_CP009273/4501052‑4501294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑