Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 4,545,596 T→C E181G (GAG→GGG)  yjiC ← YjfZ/YjiC family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,545,5960TC100.0% 143.6 / NA 39E181G (GAG→GGG) yjiCYjfZ/YjiC family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/17);  total (22/17)

GGCTTCTATACCAATTATTGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCG  >  NZ_CP009273/4545460‑4545727
                                                                                                                                        |                                                                                                                                   
ggCTTCTATACCAATTATTGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCa                                                                                                                                   >  1:192988/1‑139 (MQ=255)
 gCTTCTATACCAATTATTGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCAt                                                                                                                                  <  2:301255/139‑1 (MQ=255)
                          tACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGc                                                                                                         >  2:86414/1‑139 (MQ=255)
                                aCTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAg                                                                                                   >  1:433624/1‑139 (MQ=255)
                                aCTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAg                                                                                                   >  2:131133/1‑139 (MQ=255)
                                aCTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCCGAGAAGCTGGAAg                                                                                                   >  2:94175/1‑139 (MQ=255)
                                 cTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAgg                                                                                                  >  1:286806/1‑139 (MQ=255)
                                    aaTATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGtt                                                                                               >  2:344354/1‑139 (MQ=255)
                                      tatTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTg                                                                                              >  2:369928/1‑138 (MQ=255)
                                           gCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAata                                                                                         >  1:132076/1‑138 (MQ=255)
                                              ttAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATc                                                                                     >  2:407609/1‑139 (MQ=255)
                                                aaGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAggg                                                                                                 >  2:414899/1‑125 (MQ=255)
                                                aaGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAggg                                                                                                 <  1:414899/125‑1 (MQ=255)
                                                      tGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGa                                                                                                     <  1:116089/115‑1 (MQ=255)
                                                      tGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGa                                                                                                     >  2:116089/1‑115 (MQ=255)
                                                                               tttGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGtt                                                                                               <  1:89172/96‑1 (MQ=255)
                                                                               tttGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGtt                                                                                               >  2:89172/1‑96 (MQ=255)
                                                                                   cTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAAc                                                 <  2:192988/138‑1 (MQ=255)
                                                                                      ccccTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCa                                               <  2:181342/137‑1 (MQ=255)
                                                                                                    aTTATCTGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTAttt                               >  2:359384/1‑139 (MQ=255)
                                                                                                          tGATCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGaa                         >  2:231227/1‑139 (MQ=255)
                                                                                                            aTCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATg                           <  2:433624/135‑1 (MQ=255)
                                                                                                            aTCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGaa                         <  1:94175/137‑1 (MQ=255)
                                                                                                            aTCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGaa                         <  1:131133/137‑1 (MQ=255)
                                                                                                            aTCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGaa                         <  1:359384/137‑1 (MQ=255)
                                                                                                            aTCAATATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGaa                         <  1:369928/137‑1 (MQ=255)
                                                                                                               aaTATAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGta                    <  2:177035/139‑1 (MQ=255)
                                                                                                                atatAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGa                                                           <  2:145953/99‑1 (MQ=255)
                                                                                                                atatAGATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGa                                                           >  1:145953/1‑99 (MQ=255)
                                                                                                                     gATATCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTc               <  2:404934/138‑1 (MQ=255)
                                                                                                                      atatCAATCCTTGGCTGCCCCTTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATg                           >  1:53745/1‑125 (MQ=255)
                                                                                                                      atatCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCa                                                                    <  2:345542/84‑1 (MQ=255)
                                                                                                                      atatCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCa                                                                    >  1:345542/1‑84 (MQ=255)
                                                                                                                      atatCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATg                           <  2:53745/125‑1 (MQ=255)
                                                                                                                        atCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTc            >  2:239420/1‑138 (MQ=255)
                                                                                                                         tCAATCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCaa          >  2:112729/1‑139 (MQ=255)
                                                                                                                            aTCCTTGGCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAg       <  1:103984/139‑1 (MQ=255)
                                                                                                                                  ggCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCg  >  1:318601/1‑138 (MQ=255)
                                                                                                                                  ggCTGCCCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCg  >  2:350933/1‑138 (MQ=255)
                                                                                                                                        |                                                                                                                                   
GGCTTCTATACCAATTATTGGGTCATTACCCAACTCAATATTAGCATTAAGTAATGCGGCAAGGGCGAATCCGGAAGAATTTGCTACCCCTGTTACCGTCATTATCTGATCAATATAGATATCAATCCTTGGCTGCTCATTGGTCCATCTTTTTGCCTGAGAAGCTGGAAGGGTTGAAATATATCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCG  >  NZ_CP009273/4545460‑4545727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑