| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,568,721 | A→G | I145T (ATC→ACC) | mcrB ← | 5‑methylcytosine‑specific restriction endonuclease subunit McrB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,568,721 | 0 | A | G | 100.0% | 89.5 / NA | 25 | I145T (ATC→ACC) | mcrB | 5‑methylcytosine‑specific restriction endonuclease subunit McrB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/11); total (14/11) | |||||||||||
TTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGATTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGATTGTTT > NZ_CP009273/4568588‑4568856 | tttGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTAtg < 2:308003/139‑1 (MQ=255)tttGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTAtg < 2:221628/139‑1 (MQ=255) tCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCggg > 2:396533/1‑139 (MQ=255) ccAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTggg > 1:313905/1‑138 (MQ=255) cAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGaa < 2:201760/139‑1 (MQ=255) tATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAAc > 2:89205/1‑133 (MQ=255) tATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAAc < 1:89205/133‑1 (MQ=255) tATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCg > 2:147346/1‑138 (MQ=255) tATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCg > 2:324711/1‑138 (MQ=255) tttttGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAgg > 2:203395/1‑126 (MQ=255) tttttGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAgg < 1:203395/126‑1 (MQ=255) tttttGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTg > 2:100127/1‑139 (MQ=255) tttttGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTg > 1:286754/1‑139 (MQ=255) ggTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTAtttt < 2:150457/138‑1 (MQ=255) tGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTa < 1:324711/139‑1 (MQ=255) acacTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGatat < 1:322370/138‑1 (MQ=255) tCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAAc < 2:205110/70‑1 (MQ=255) tCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAAc > 1:205110/1‑70 (MQ=255) gTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGATTGtt > 2:24246/1‑139 (MQ=255) gTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGATTGtt > 2:390664/1‑139 (MQ=255) gTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACATTTTGGGAACAgg < 1:400295/71‑1 (MQ=255) gTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACATTTTGGGAACAgg > 2:400295/1‑71 (MQ=255) ttGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGa < 2:124399/133‑1 (MQ=255) ttGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGa > 1:124399/1‑133 (MQ=255) ttGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGATTGttt > 1:428889/1‑139 (MQ=255) | TTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGATTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGATTGTTT > NZ_CP009273/4568588‑4568856 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |