Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	509,698	A→C	T84P (ACG→CCG)	cueR →	Cu(I)‑responsive transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	509,698	0	A	C	100.0%	58.6 / NA	18	T84P (ACG→CCG)	cueR	Cu(I)‑responsive transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (11/7);  total (11/7)

AGCAGCATCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCT  >  NZ_CP009273/509570‑509827                                                                                                                                 |                                                                                                                                  agcagcaTCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGgag                                                                                                                           >  2:329623/1‑137 (MQ=255) agcagcaTCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGgag                                                                                                                           >  2:143861/1‑137 (MQ=255)  gcagcaTCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAg                                                                                                                        <  1:174753/139‑1 (MQ=255)        tctcAACGAACTGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCg                                                                                                                  >  1:119780/1‑139 (MQ=255)                    gACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGa                                                                                                      >  2:245696/1‑139 (MQ=255)                     aCCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACg                                                                                                       <  1:30704/137‑1 (MQ=255)                             gcgcCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTgag                                                                                             <  2:41427/139‑1 (MQ=255)                                ccAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTgagg                                                                                            >  2:430213/1‑137 (MQ=255)                                ccAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTgagg                                                                                            <  1:430213/137‑1 (MQ=255)                                 cAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGc                                                                                         >  1:212419/1‑139 (MQ=255)                                             gTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATgc                                                                             >  1:388390/1‑139 (MQ=255)                                                               gagagCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCAc                                                           <  1:329623/139‑1 (MQ=255)                                                                  agCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAAc                                                                                                        <  1:36508/91‑1 (MQ=255)                                                                  agCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAAc                                                                                                        >  2:36508/1‑91 (MQ=255)                                                                  agCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTgg                                                        >  2:419041/1‑139 (MQ=255)                                                                              gTGAATCAGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAAtgcctgcc                                            <  1:337580/139‑1 (MQ=255)                                                                                                    aGCGGCACAGCGCCGACGTCAAACGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTg                      >  2:185408/1‑139 (MQ=255)                                                                                                                        aaaCGGCGCCCGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAAtctct  >  1:367343/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                  AGCAGCATCTCAACGAACTGACCTTACTGCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCT  >  NZ_CP009273/509570‑509827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑