Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	552,665	T→C	E178G (GAA→GGA)	folD ←	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	552,665	0	T	C	100.0%	106.3 / NA	29	E178G (GAA→GGA)	folD	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/13);  total (16/13)

CAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGACGCGGCGCGCG  >  NZ_CP009273/552539‑552798                                                                                                                               |                                                                                                                                      cAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATc                                                                                                                            <  1:33678/138‑1 (MQ=255)    tCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGggc                                                                                                                        >  2:361409/1‑139 (MQ=255)                     tGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGAt                                                                                                       >  2:57391/1‑139 (MQ=255)                       gCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATgc                                                                                                     >  2:429708/1‑139 (MQ=255)                        cTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATgcg                                                                                                    <  1:361409/139‑1 (MQ=255)                            ccAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCa                                                                                                 <  2:123521/138‑1 (MQ=255)                                 ggCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATcac                                                                                            >  2:340345/1‑138 (MQ=255)                                             tAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAgg                                                                               >  1:383939/1‑139 (MQ=255)                                                aTCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGcc                                                                             >  1:289713/1‑138 (MQ=255)                                                 tCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGa                                                                           <  1:351593/139‑1 (MQ=255)                                                  cGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCg                                                                            >  1:22134/1‑137 (MQ=255)                                                  cGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCg                                                                            >  1:378826/1‑137 (MQ=255)                                                       ttttCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTAt                                                                     <  1:87372/139‑1 (MQ=255)                                                              cGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAAtgtt                                                              >  2:303977/1‑139 (MQ=255)                                                                tgatgaCGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAAtgttgt                                                            <  2:53783/139‑1 (MQ=255)                                                                  atgaCGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTaa                                                          <  2:107583/139‑1 (MQ=255)                                                                  atgaCGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTaa                                                          >  1:371725/1‑139 (MQ=255)                                                                                              gTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACcgcgcg                              <  1:392960/139‑1 (MQ=255)                                                                                              gTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACcgcgcg                              <  2:29350/139‑1 (MQ=255)                                                                                              gTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACcgcgcg                              <  2:378826/139‑1 (MQ=255)                                                                                                  cTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACcgcgcg                              <  2:88806/135‑1 (MQ=255)                                                                                                  cTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACcgcgcg                              >  1:88806/1‑135 (MQ=255)                                                                                                       gtggtgCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGcc                                                                             <  2:360297/83‑1 (MQ=255)                                                                                                       gtggtgCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGcc                                                                             >  1:360297/1‑83 (MQ=255)                                                                                                       gtggtgCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAgg                     >  2:183390/1‑139 (MQ=255)                                                                                                             cAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACg                 >  2:301277/1‑137 (MQ=255)                                                                                                                  tGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGAcgc          >  2:382235/1‑139 (MQ=255)                                                                                                                     cagcagcagTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGACgcggc       >  1:248478/1‑139 (MQ=255)                                                                                                                          gcagTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGACGCGgcgcgcg  <  2:383939/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                      CAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGACGCGGCGCGCG  >  NZ_CP009273/552539‑552798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑