| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 579,220 | T→A | N28K (AAT→AAA) | appY → | DNA‑binding transcriptional activator AppY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 579,220 | 0 | T | A | 100.0% | 23.4 / NA | 7 | N28K (AAT→AAA) | appY | DNA‑binding transcriptional activator AppY |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (4/3); total (4/3) | |||||||||||
ACTACTATCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATGTTTGCTCCGTAGTTTTCATCTGTCAATCATTTGATTTAATTATAAACAGGAGAGTTATCTCGTTCAAAAAAAATTCATTGTTTATTGTAAGCGACAAAATTAGAAGGGAGTTACCAGTATGCCCCTCTAAACTAAGAATTGTTGATATAGATAAGAAAACATGTTTATCCTTTTTTATCGACGTGAATAATGAGCTGCCTG > NZ_CP009273/579098‑579347 | actactATCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATGTTTGCTCCGTAGTTTTCATCTGTCAATCATTTGATTTAATTATAAACAGGAGAGTTATCTCGTTCAAAAAAAAATCATTGTTTATTGt > 2:184624/1‑137 (MQ=255) tATGATAGGTGCAAGATGGATTATGTTTGCTCCGTAGTTTTCATCTGTCAATCATTTGATTTAATTATAAACAGGAGAGTTATCTCGTTCAAAAAAAAATCATTGTTTATTGTAAGCGACAAAATTAGAAGGGAGTTa < 2:28778/138‑1 (MQ=255) gATGGATTATGTTTGCTCCGTAGTTTTCATCTGTCAATCATTTGATTTAATTATAAACAGGAGAGTTATCTCGTTCAAAAAAAAATCATTGTTTATTGTAAGCGACAAAATTAGAAGGGAGTTACCAGTATGCCCctct < 1:184624/139‑1 (MQ=255) tGTTTGCTCCGTAGTTTTCATCTGTCAATCATTTGATTTAATTATAAACAGGAGAGTTATCTCGTTCAAAAAAAAATCATTGTTTATTGTAAGCGACAAAATTAGAAGGGAGTTACCAGTATGCCCCTCTAAACTAAGa < 1:169301/139‑1 (MQ=255) gCTCCGTAGTTTTCATCTGTCAATCATTTGATTTAATTATAAACAGGAGAGTTATCTCGTTCAAAAAAAAATCATTGTTTATTGTAAGCGACAAAATTAGAAGGGAGTTACCAGTATGCCCCTCTAAACTAAGAAttgt > 1:132390/1‑139 (MQ=255) gagaGTTATCTCGTTCAAAAAAAAATCATTGTTTATTGTAAGCGACAAAATTAGAAGGGAGTTACCAGTATGCCCCTCTAAACTAAGAATTGTTGATATAGATAAGAAAATATGTTTATCCTTTTTTATCGACGTGaa > 2:123019/1‑138 (MQ=255) ttCAAAAAAAAATCATTGTTTATTGTAAGCGACAAAATTAGAAGGGAGTTACCAGTATGCCCCTCTAAACTAAGAATTGTTGATATAGATAAGAAAACATGTTTATCCTTTTTTATCGACGTGAATAATGAGCTGCCTg > 1:71574/1‑139 (MQ=255) | ACTACTATCAACTTGTTTTAATTTTATGATAGGTGCAAGATGGATTATGTTTGCTCCGTAGTTTTCATCTGTCAATCATTTGATTTAATTATAAACAGGAGAGTTATCTCGTTCAAAAAAAATTCATTGTTTATTGTAAGCGACAAAATTAGAAGGGAGTTACCAGTATGCCCCTCTAAACTAAGAATTGTTGATATAGATAAGAAAACATGTTTATCCTTTTTTATCGACGTGAATAATGAGCTGCCTG > NZ_CP009273/579098‑579347 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |