Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	715,111	A→G	I269T (ATC→ACC)	speF ←	ornithine decarboxylase SpeF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	715,111	0	A	G	100.0%	94.5 / NA	27	I269T (ATC→ACC)	speF	ornithine decarboxylase SpeF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/16);  total (11/16)

CACAGATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGATCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTCCGTGGTGGTT  >  NZ_CP009273/714985‑715238                                                                                                                               |                                                                                                                                cacaGATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACg                                                                                                                       >  2:386140/1‑137 (MQ=255)   caGATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAgg                                                                                                                   <  1:42376/139‑1 (MQ=255)     gATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGt                                                                                                                  >  2:271807/1‑138 (MQ=255)         aCCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACt                                                                                                              <  1:271807/138‑1 (MQ=255)          ccAATTTTATCCACCACTGGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACttt                                                                                                            <  2:241345/139‑1 (MQ=255)             aTTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTttctt                                                                                                         <  1:287292/139‑1 (MQ=255)                   tCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCaaaa                                                                                                    <  2:90908/138‑1 (MQ=255)                   tCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAAGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCCGGTAACTTTCTTCaaaa                                                                                                    >  1:40052/1‑138 (MQ=255)                       ccacTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTg                                                                                               <  1:386140/139‑1 (MQ=255)                        cacTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTgc                                                                                              <  2:352152/139‑1 (MQ=255)                                             cGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAg                                                                         >  2:342027/1‑139 (MQ=255)                                                   gtcgtAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATAc                                                                   <  2:231225/139‑1 (MQ=255)                                                    tcgtAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACg                                                                  >  1:418833/1‑139 (MQ=255)                                                             ccTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTg                                                         <  2:157247/139‑1 (MQ=255)                                                              cTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGc                                                        >  2:189694/1‑139 (MQ=255)                                                                             cGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAAc                                         >  2:378853/1‑139 (MQ=255)                                                                              gAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAAcc                                        <  2:356756/139‑1 (MQ=255)                                                                                  cGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGgtg                                    <  1:83216/139‑1 (MQ=255)                                                                                            gAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGcc                                                                                   >  2:255713/1‑82 (MQ=255)                                                                                            gAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGcc                                                                                   <  1:255713/82‑1 (MQ=255)                                                                                                            cTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTCCg          <  1:378853/139‑1 (MQ=255)                                                                                                             tGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTCCgt         >  1:263561/1‑139 (MQ=255)                                                                                                             tGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAAC‑TTCTTCAAAACAGTGCGCATCAATGCCACCGATa                                                                            <  2:27348/71‑1 (MQ=255)                                                                                                             tGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAAC‑TTCTTCAAAACAGTGCGCATCAATGCCACCGATa                                                                            >  1:27348/1‑71 (MQ=255)                                                                                                               cGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTCCgtg        <  1:23368/138‑1 (MQ=255)                                                                                                                  tGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTCCGtggtgg    >  1:195304/1‑139 (MQ=255)                                                                                                                    cGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTCCGTGGTGGtt  <  1:205825/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                CACAGATGACCAATTTTATCCACCACTTGGCGGGCGTTATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGATCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGTGCCGTTTCCAGATAAACCGGTGTTGCACCAGCCTGTAGCAACGCTCCGTGGTGGTT  >  NZ_CP009273/714985‑715238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑