Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	857,539	T→C	E175G (GAA→GGA)	ybiW ←	glycyl radical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	857,539	0	T	C	100.0%	107.3 / NA	30	E175G (GAA→GGA)	ybiW	glycyl radical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/15);  total (15/15)

GACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAGCGATCCTGTACGGTC  >  NZ_CP009273/857415‑857673                                                                                                                             |                                                                                                                                       gactgCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCgg                                                                                                                           >  1:77209/1‑138 (MQ=255)  actgCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCggg                                                                                                                          >  2:201500/1‑138 (MQ=255)              aCGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCa                                                                                                             <  1:385834/139‑1 (MQ=255)                       aTCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCAt                                                                                                    >  2:379717/1‑139 (MQ=255)                       aTCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCAt                                                                                                    >  1:131498/1‑139 (MQ=255)                            tttCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCg                                                                                               >  2:322438/1‑139 (MQ=255)                            tttCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCg                                                                                               >  1:321100/1‑139 (MQ=255)                             ttCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCa                                                                                                                   >  1:14839/1‑118 (MQ=255)                             ttCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCa                                                                                                                   <  2:14839/118‑1 (MQ=255)                              tCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCg                                                                                               <  2:377810/137‑1 (MQ=255)                                        cTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTg                                                                                   >  2:438336/1‑139 (MQ=255)                                           aCCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCt                                                                                <  1:322438/139‑1 (MQ=255)                                            ccGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCtt                                                                               <  1:18480/139‑1 (MQ=255)                                             cGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTc                                                                              <  2:138742/139‑1 (MQ=255)                                                                 gTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCg                                                          >  2:330483/1‑139 (MQ=255)                                                                  tCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGc                                                         >  2:288563/1‑139 (MQ=255)                                                                          aTGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGAc                                                 >  1:166518/1‑139 (MQ=255)                                                                                gagcgaCGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCttttt                                           >  2:1183/1‑139 (MQ=255)                                                                                 agcgaCGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTg                                          >  1:15340/1‑139 (MQ=255)                                                                                   cgaCGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCt                                        <  1:407023/139‑1 (MQ=255)                                                                                   cgaCGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCt                                        <  2:166518/139‑1 (MQ=255)                                                                                   cgaCGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCt                                        <  1:379717/139‑1 (MQ=255)                                                                                           cGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGt                                 <  2:240047/138‑1 (MQ=255)                                                                                           cGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGt                                 <  1:308224/138‑1 (MQ=255)                                                                                                cTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACa                            <  2:356835/138‑1 (MQ=255)                                                                                                cTTCCTCGCGCAGACCATCAAGCCC‑TTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATg                          >  2:14799/1‑139 (MQ=255)                                                                                                cTTCCCCGCGCCGCCCATCAAGCCCTTTTCCCAGAACCACCGGGAAATTCACCGCCAGGTGCGATTCACCGGAGGTCATATTGCCTTCCGCTTTAACGATTCGGGTCGCCAGCAGACCCTTTTGCTACTCGGGAAACa                            <  2:435667/138‑1 (MQ=255)                                                                                                              ccATCAAGCCCTTCTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAGCGAt             <  2:77209/139‑1 (MQ=255)                                                                                                                  cAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTagcag                 <  2:286018/131‑1 (MQ=255)                                                                                                                  cAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTagcag                 >  1:286018/1‑131 (MQ=255)                                                                                                                         ttttcCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAGCGATCCTGTACGGTc  >  1:148784/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                       GACTGCCACCAGCACGATATCAATCGCTTTCAGGAATTGCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAATGATTCCGGTCGCCAGCAGACCTTTTTGCTCATCGGTAAACATGCCGTAGCAGCGATCCTGTACGGTC  >  NZ_CP009273/857415‑857673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑