Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	890,897	T→C	S151P (TCC→CCC)	potG →	putrescine ABC transporter ATP‑binding subunit PotG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	890,897	0	T	C	100.0%	69.0 / NA	20	S151P (TCC→CCC)	potG	putrescine ABC transporter ATP‑binding subunit PotG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/11);  total (9/11)

TGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGACAGGATGCAGCTTGAAGTGGTGG  >  NZ_CP009273/890763‑891026                                                                                                                                       |                                                                                                                                  tGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGAGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCg                                                                                                                                <  1:4585/138‑1 (MQ=255)     cGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCggtggt                                                                                                                           <  2:347708/139‑1 (MQ=255)       tGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCggtggt                                                                                                                           >  1:328924/1‑137 (MQ=255)                  aTCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACgtg                                                                                                              <  2:238578/139‑1 (MQ=255)                  aTCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACgtg                                                                                                              <  2:166104/139‑1 (MQ=255)                    cGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGtgtg                                                                                                            >  1:251878/1‑139 (MQ=255)                         ttGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGtggccc                                                                                                        <  2:306253/138‑1 (MQ=255)                                       gACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTg                                                                                         >  1:91960/1‑139 (MQ=255)                                            aCTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAg                                                                                    >  2:427527/1‑139 (MQ=255)                                                ccGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGcc                                                                                <  2:251878/139‑1 (MQ=255)                                                                    ccGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGAt                                                            <  1:380951/139‑1 (MQ=255)                                                                                  tGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGAt                                                    >  1:441174/1‑133 (MQ=255)                                                                                    cTNGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGgcgcg                                             <  2:441174/138‑1 (MQ=255)                                                                                    cTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGgcgcgc                                            <  1:266307/139‑1 (MQ=255)                                                                                     tCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCt                                           >  2:269233/1‑139 (MQ=255)                                                                                                   atGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGc                             >  1:349270/1‑139 (MQ=255)                                                                                                        ggAGTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGa                         >  1:217779/1‑138 (MQ=255)                                                                                                           gTTCGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGACAg                      >  2:303785/1‑138 (MQ=255)                                                                                                              cGCCAAACGCAAACCGCATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGACAGGAt                   <  1:269233/138‑1 (MQ=255)                                                                                                                              cATCAGCTTCCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGACAGGATGCAGCTTGAAGtggtgg  <  2:217779/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                  TGACCGTGGAACAGAACATCGCTTTTGGCCTGAAACAGGACAAACTACCGAAAGCGGAAATTGCCAGCCGGGTCAATGAGATGCTCGGCCTGGTGCATATGCAGGAGTTCGCCAAACGCAAACCGCATCAGCTTTCCGGTGGTCAGCGACAACGTGTGGCCCTGGCCCGAAGCCTTGCGAAGCGCCCGAAACTATTACTGCTCGATGAGCCGATGGGCGCGCTGGATAAAAAGCTGCGTGACAGGATGCAGCTTGAAGTGGTGG  >  NZ_CP009273/890763‑891026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑