Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	900,176	T→G	V43G (GTT→GGT)	ybjQ →	heavy metal‑binding domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	900,176	0	T	G	100.0%	63.7 / NA	19	V43G (GTT→GGT)	ybjQ	heavy metal‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (10/9);  total (10/9)

CGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCCGTCGTCGGTATTGATAT  >  NZ_CP009273/900044‑900293                                                                                                                                     |                                                                                                                      cgatcATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCg                                                                                                                  >  1:284597/1‑138 (MQ=255)                   cAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTa                                                                                                  <  2:291261/136‑1 (MQ=255)                          aaCTCTGGAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGaa                                                                                        <  1:130793/139‑1 (MQ=255)                          aaCTCTGGAAGGCCACACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTAGGAAAAAGaa                                                                                        <  1:335663/139‑1 (MQ=255)                           aCTCTGGAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAAc                                                                                       >  2:285184/1‑139 (MQ=255)                                 gAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGt                                                                                  >  1:279336/1‑138 (MQ=255)                                 gAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGAGTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCg                                                                                   <  1:190094/137‑1 (MQ=255)                                       cAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCAc                                                                           >  1:178824/1‑139 (MQ=255)                                       cAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCAc                                                                           >  2:30448/1‑139 (MQ=255)                                                        ttGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAg                                                          >  1:125341/1‑139 (MQ=255)                                                           cGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGaa                                                       >  1:204493/1‑139 (MQ=255)                                                                tggtgACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAgg                                                  <  2:279336/139‑1 (MQ=255)                                                                tggtgACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATAGCGGTGGCGGACGTTCCGGTGCGTATGAACAAGAACTGCGTAAAGCACGGGAGCTCGCCTTTGAGGAATTAg                                                   <  1:145034/138‑1 (MQ=255)                                                                        ggCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAgg                                          >  1:381299/1‑139 (MQ=255)                                                                                                            ttCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCCgtcgtc             <  1:257236/132‑1 (MQ=255)                                                                                                            ttCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCCgtcgtc             >  2:257236/1‑132 (MQ=255)                                                                                                                 tGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCCGTCGTCGGTATTGatat  <  2:204493/138‑1 (MQ=255)                                                                                                                                 atCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCCGTCGTCGGTa         <  1:235247/115‑1 (MQ=255)                                                                                                                                 atCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCCGTCGTCGGTa         >  2:235247/1‑115 (MQ=255)                                                                                                                                     |                                                                                                                      CGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGGCGCGGGCGCTGGGGGCCGATGCCGTCGTCGGTATTGATAT  >  NZ_CP009273/900044‑900293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑