Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	928,200	T→C	R50R (CGT→CGC)	lrp →	leucine‑responsive transcriptional regulator Lrp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	928,200	0	T	C	100.0%	56.3 / NA	18	R50R (CGT→CGC)	lrp	leucine‑responsive transcriptional regulator Lrp
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/12);  total (6/12)

GAAGCGCCCTGGCAAAGATCTCGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTG‑CCTTGAGCGTGTGCGTCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTGGCGCACCGGATGTGTTTGAACAATTCAATA  >  NZ_CP009273/928065‑928333                                                                                                                                         |                                                                                                                                      gaagCGCCCTGGCAAAGATCTCGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTG‑CCTTGAGCGTGTGCGCCgg                                                                                                                                    <  1:351722/139‑1 (MQ=255)            gCAAAGATCTCGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTG‑CCTTGAGCGTGTGCGCCGGCTGGAAAGACa                                                                                                                         <  1:163089/139‑1 (MQ=255)                 gATCTCGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTG‑CCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGt                                                                                                                    <  1:130348/139‑1 (MQ=255)                 gATCTCGACCGTATCGATCGTAACATTCTTAATGAGGTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTG‑CCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGt                                                                                                                    <  2:298918/139‑1 (MQ=255)                        aCCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTG‑CCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCa                                                                                                             <  2:417277/139‑1 (MQ=255)                                             ttAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTG‑CCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTaa                                                                                        >  1:280238/1‑139 (MQ=255)                                                   aGTTTCGAAAGGCTGGCCGTATTTCTAACGTCGAGCTTGCTAAACGAGTGAGGCTTACCTCACGGCCTTGTCAAAGATC‑TGTGCGCCGGTTAGAAAGGCATGGGTTTATTCACGGCTATACTGCGCGGCTTAAcccag                                                                                   <  2:256784/138‑3 (MQ=255)                                                     ttGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTG‑CCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAAccccc                                                                                   <  2:87673/136‑1 (MQ=255)                                                     ttGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTG‑CCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAAccccc                                                                                   >  1:87673/1‑136 (MQ=255)                                                           aaGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTG‑CCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTgg                                                                          <  2:47598/139‑1 (MQ=255)                                                                  ggCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTG‑CCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCAt                                                                    >  2:403914/1‑138 (MQ=255)                                                                  ggCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTG‑CCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGATTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCAt                                                                    >  2:270591/1‑138 (MQ=255)                                                                                      cTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTG‑CCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTgaga                                               <  1:403914/139‑1 (MQ=255)                                                                                      cTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTG‑CCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTgaga                                               <  1:217485/139‑1 (MQ=255)                                                                                                   tgGGACTTTCCCCAACGCCGTG‑CCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCg                                  >  1:394839/1‑139 (MQ=255)                                                                                                      gACTTTCCCCAACGCCGTG‑CCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTgg                               <  2:280640/139‑1 (MQ=255)                                                                                                                          ccTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTGGCGCACCGGATGTGTTTGaa            <  2:204650/139‑1 (MQ=255)                                                                                                                                    gtgCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTGGCGCACCGGATGTGTTTGAACAATTCAATa  >  1:1291/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                      GAAGCGCCCTGGCAAAGATCTCGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTG‑CCTTGAGCGTGTGCGTCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTGGCGCACCGGATGTGTTTGAACAATTCAATA  >  NZ_CP009273/928065‑928333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑