Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	974,173	T→C	S798P (TCC→CCC)	mukB →	chromosome partition protein MukB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	974,173	0	T	C	100.0%	65.2 / NA	19	S798P (TCC→CCC)	mukB	chromosome partition protein MukB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/9);  total (10/9)

CTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGTCTGACCCG  >  NZ_CP009273/974041‑974283                                                                                                                                     |                                                                                                               cTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAAc                                                                                                          >  1:383089/1‑139 (MQ=255) cTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAAc                                                                                                          >  2:204688/1‑139 (MQ=255) cTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAAc                                                                                                          >  2:211868/1‑139 (MQ=255)        aaGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGc                                                                                                   <  1:383620/139‑1 (MQ=255)         aGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGcc                                                                                                  <  2:306648/139‑1 (MQ=255)            ggTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACg                                                                                               <  1:204574/139‑1 (MQ=255)              tAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGct                                                                                             <  2:106372/139‑1 (MQ=255)                 tggtgAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCtctc                                                                                          <  1:204688/139‑1 (MQ=255)                                      aGTGGCGTTATTCACGTGTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTAGTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAAcgc                                                                      <  2:244588/138‑3 (MQ=255)                                             ttATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACt                                                                       <  2:63449/130‑1 (MQ=255)                                             ttATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACt                                                                       >  1:282769/1‑130 (MQ=255)                                             ttATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACt                                                                       >  1:63449/1‑130 (MQ=255)                                             ttATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACt                                                                       <  2:282769/130‑1 (MQ=255)                                             ttATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTg                                                              >  1:151125/1‑139 (MQ=255)                                                        tCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGtt                                                   >  1:82188/1‑139 (MQ=255)                                                          ccGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGACACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGtt                                                   >  1:268925/1‑137 (MQ=255)                                                                                                          aGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTATGCGTTTGACCCg  >  2:232862/1‑138 (MQ=255)                                                                                                                       aGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGtt                    >  2:195619/1‑107 (MQ=255)                                                                                                                       aGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGtt                    <  1:195619/107‑1 (MQ=255)                                                                                                                                     |                                                                                                               CTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGTCTGACCCG  >  NZ_CP009273/974041‑974283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑