Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,079,189	T→C	S120P (TCC→CCC)	efeB →	iron uptake transporter deferrochelatase/peroxidase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,079,189	0	T	C	100.0%	60.2 / NA	18	S120P (TCC→CCC)	efeB	iron uptake transporter deferrochelatase/peroxidase subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/12);  total (6/12)

GCGTTTGATGTGCTTGCCAGCGATAAAGCCGATCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATTCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGATGCCAAAAAAG  >  NZ_CP009273/1079057‑1079299                                                                                                                                     |                                                                                                               gCGTTTGATGTGCTTGCCAGCGATAAAGCCGATCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGc                                                                                                           <  1:345876/138‑1 (MQ=255)    tttGATGTGCTTGCCAGCGATAAAGCCGATCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTc                                                                                                       >  1:208426/1‑139 (MQ=255)           tgCTTGCCAGCGATAAAGCCGATCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTggcgg                                                                                                <  2:208426/139‑1 (MQ=255)                gCCAGCGATAAAGCCGATCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACa                                                                                           >  2:164607/1‑139 (MQ=255)                               gATCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAAtc                                                                            >  1:146097/1‑139 (MQ=255)                                     gAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCAcc                                                                       <  2:267663/138‑1 (MQ=255)                                     gAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCAc                                                                        <  2:146097/137‑1 (MQ=255)                                      aGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCAt                                                                     <  2:143121/139‑1 (MQ=255)                                      aGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCAt                                                                     <  1:414496/139‑1 (MQ=255)                                             gttTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCtt                                                                                                     >  2:133423/1‑100 (MQ=255)                                             gttTCGCTTGTTGACTCAGAGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCtt                                                                                                     <  1:133423/100‑1 (MQ=255)                                                  gCTTGTTGGCTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGt                                                         <  2:288141/139‑1 (MQ=255)                                                           cTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTc                                                <  2:379533/139‑1 (MQ=255)                                                                cGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGt                                           >  1:249867/1‑139 (MQ=255)                                                                       cTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTtgatg                                     <  2:249867/138‑1 (MQ=255)                                                                                                    gAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCAt                                                                     >  2:241188/1‑77 (MQ=255)                                                                                                    gAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCAt                                                                     <  1:241188/77‑1 (MQ=255)                                                                                                         gCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGATGCCAAAAAAg  <  2:418821/139‑1 (MQ=255)                                                                                                                                     |                                                                                                               GCGTTTGATGTGCTTGCCAGCGATAAAGCCGATCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATTCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGATGCCAAAAAAG  >  NZ_CP009273/1079057‑1079299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑