Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,097,764	A→G	N78N (AAT→AAC)	csgE ←	curli production assembly/transport protein CsgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,097,764	0	A	G	100.0%	79.6 / NA	22	N78N (AAT→AAC)	csgE	curli production assembly/transport protein CsgE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/16);  total (6/16)

TCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAA  >  NZ_CP009273/1097630‑1097890                                                                                                                                       |                                                                                                                               tCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGa                                                                                                                            <  1:347471/139‑1 (MQ=255)   gCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGAc                                                                                                                           <  1:404637/138‑1 (MQ=255)    cccGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCg                                                                                                                         >  2:423312/1‑139 (MQ=255)             aataaCGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTg                                                                                                                 <  1:26057/138‑1 (MQ=255)              ataaCGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGAt                                                                                                               <  2:357454/139‑1 (MQ=255)              ataaCGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGAt                                                                                                               <  1:441023/139‑1 (MQ=255)                 aCGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATcc                                                                                                             <  2:153976/138‑1 (MQ=255)                    ccTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCt                                                                                                         >  1:223031/1‑139 (MQ=255)                        aTTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTcc                                                                                                      <  2:298152/138‑1 (MQ=255)                             tCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATc                                                                                                 <  2:326660/138‑1 (MQ=255)                             tCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCg                                                                                                >  1:97424/1‑139 (MQ=255)                                   gacgaTTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACt                                                                                          <  1:143244/139‑1 (MQ=255)                                    acgaTTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTg                                                                                         <  1:4638/139‑1 (MQ=255)                                             tGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCa                                                                                >  2:135564/1‑139 (MQ=255)                                                             aTCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTAc                                                                <  1:107003/139‑1 (MQ=255)                                                                            acAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACttt                                                 >  2:380616/1‑139 (MQ=255)                                                                                       gAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTa                                         <  2:223031/136‑1 (MQ=255)                                                                                                 ttCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAgg                               <  1:108057/136‑1 (MQ=255)                                                                                                              aaaaaGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATg               <  2:301969/139‑1 (MQ=255)                                                                                                                  aGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCa           <  1:423312/139‑1 (MQ=255)                                                                                                                   gTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCaa          >  2:10013/1‑139 (MQ=255)                                                                                                                           aaTAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGaa  <  1:135564/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                               TCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAA  >  NZ_CP009273/1097630‑1097890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑