| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,124,635 | T→C | R447R (CGT→CGC) | murJ → | murein biosynthesis integral membrane protein MurJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,124,635 | 0 | T | C | 100.0% | 52.8 / NA | 16 | R447R (CGT→CGC) | murJ | murein biosynthesis integral membrane protein MurJ |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (7/9); total (7/9) | |||||||||||
CGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTG > NZ_CP009273/1124505‑1124762 | cGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCtggtggt > 2:440219/1‑139 (MQ=255) ttGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCtggtggtgg < 1:120494/139‑1 (MQ=255) ttGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCtggtggtgg > 2:188680/1‑139 (MQ=255) cTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCg < 1:188680/139‑1 (MQ=255) cTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCg < 1:89613/139‑1 (MQ=255) gTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAg < 2:271837/138‑1 (MQ=255) tgtCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACata < 1:210120/139‑1 (MQ=255) tgtCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACata < 1:282959/139‑1 (MQ=255) tCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGt < 1:90662/95‑1 (MQ=255) tCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGt > 2:90662/1‑95 (MQ=255) tCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGt > 1:246556/1‑139 (MQ=255) cGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTg > 1:210929/1‑139 (MQ=255) tACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCAtt < 1:91421/137‑1 (MQ=255) aGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCtggtggt < 1:130551/55‑1 (MQ=255) aGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCtggtggt > 2:130551/1‑55 (MQ=255) tttCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTTGTGGTGGCggggggtg > 2:112552/1‑135 (MQ=255) | CGTTGAAACATGCCGGGCTGTCACTTTCTATTGGTCTGGCGGCGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTG > NZ_CP009273/1124505‑1124762 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |