Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,136,234 A→G E249G (GAA→GGA)  flgL → flagellar hook‑associated protein FlgL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,136,2340AG100.0% 81.5 / NA 23E249G (GAA→GGA) flgLflagellar hook‑associated protein FlgL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/12);  total (11/12)

TGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATG  >  NZ_CP009273/1136106‑1136364
                                                                                                                                |                                                                                                                                  
tGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCa                                                                                                                            >  2:4743/1‑137 (MQ=255)
tGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCa                                                                                                                            <  1:4743/137‑1 (MQ=255)
tGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACg                                                                                                                          >  2:5084/1‑139 (MQ=255)
                     gCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCg                                                                                                     <  1:395855/139‑1 (MQ=255)
                         gTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTgg                                                                                                 <  2:255781/139‑1 (MQ=255)
                         gTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTg                                                                                                  >  2:363897/1‑138 (MQ=255)
                          tCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAg                                                                                                        <  1:161462/131‑1 (MQ=255)
                          tCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAg                                                                                                        >  2:161462/1‑131 (MQ=255)
                                              aaaGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATcgcg                                                                            >  2:390874/1‑139 (MQ=255)
                                                                           aaccaaccGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGAt                                               <  2:88620/139‑1 (MQ=255)
                                                                                 ccGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCa                                                                                                                            <  2:136016/56‑1 (MQ=255)
                                                                                 ccGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCa                                                                                                                            >  1:136016/1‑56 (MQ=255)
                                                                                 ccGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGtt                                         >  1:223892/1‑139 (MQ=255)
                                                                                           aaaaaCTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGAc                                <  1:390874/138‑1 (MQ=255)
                                                                                           aaaaaCTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACt                               >  1:213541/1‑139 (MQ=255)
                                                                                                         cAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTAtt                 <  1:363897/139‑1 (MQ=255)
                                                                                                         cAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTAtt                 <  2:190791/139‑1 (MQ=255)
                                                                                                         cAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTAtt                 <  2:338368/139‑1 (MQ=255)
                                                                                                             gtgCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAAt                          >  1:259659/1‑126 (MQ=255)
                                                                                                             gtgCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAAt                          <  2:259659/126‑1 (MQ=255)
                                                                                                              tgCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATc            <  2:213541/139‑1 (MQ=255)
                                                                                                                      gTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTAcatca    >  1:296280/1‑139 (MQ=255)
                                                                                                                        tCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATg  >  2:273958/1‑139 (MQ=255)
                                                                                                                                |                                                                                                                                  
TGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATG  >  NZ_CP009273/1136106‑1136364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑