Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,149,506	T→C	S251P (TCC→CCC)	pabC →	aminodeoxychorismate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,149,506	0	T	C	100.0%	75.3 / NA	22	S251P (TCC→CCC)	pabC	aminodeoxychorismate lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/10);  total (12/10)

TTTGCTGGCACAATCCTCTTATCAGCTTGTCGAAGTGCAAGCCTCTCTGGAAGAGTCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCG  >  NZ_CP009273/1149376‑1149634                                                                                                                                   |                                                                                                                                 tttGCTGGCACAATCCTCTTATCAGCTTGTCGAAGTGCAAGCCTCTCTGGAAGAGTCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCg                                                                                                                          >  1:245789/1‑139 (MQ=255) tttGCTGGCACAATCCTCTTATCAGCTTGTCGAAGTGCAAGCCTCTCTGGAAGAGTCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCg                                                                                                                          >  2:2400/1‑139 (MQ=255)         cacaATCCTCTTATCAGCTTGTCGAAGTGCAAGCCTCTCTGGAAGAGTCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAAc                                                                                                                  <  2:252673/139‑1 (MQ=255)                     aTCAGCTTGTCGAAGTGCAAGCCTCTCTGGAAGAGTCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGa                                                                                                         <  1:374495/136‑1 (MQ=255)                          cTTGTCGAAGTGCAAGCCTCTCTGGAAGAGTCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAg                                                                                                 >  1:279767/1‑139 (MQ=255)                             gTCGAAGTGCAAGCCTCTCTGGAAGAGTCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGccc                                                                                              >  2:422987/1‑139 (MQ=255)                             gTCGAAGTGCAAGCCTCTCTGGAAGAGTCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGccc                                                                                              >  2:257016/1‑139 (MQ=255)                                              tctGGAAGAGTCGTTGCAGGCAGATGAGATAGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGcc                                                                               <  1:126632/137‑1 (MQ=255)                                                   aaGAGTCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAAt                                                                          >  2:293682/1‑137 (MQ=255)                                                                     atgagatgGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTa                                                      >  1:393054/1‑139 (MQ=255)                                                                                       aTGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTAtt                                     <  2:124192/138‑1 (MQ=255)                                                                                       aTGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTg                                    >  2:377219/1‑139 (MQ=255)                                                                                           gTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtgg                                <  1:377219/139‑1 (MQ=255)                                                                                            ttAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtggt                               >  2:266423/1‑139 (MQ=255)                                                                                            ttAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtggt                               >  2:293988/1‑139 (MQ=255)                                                                                              aaTGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtggtg                              <  1:2400/138‑1 (MQ=255)                                                                                                     gTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTggg                      <  2:286354/139‑1 (MQ=255)                                                                                                             cGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATcgccg              <  2:393054/139‑1 (MQ=255)                                                                                                              gTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATcgccgc             <  1:191357/139‑1 (MQ=255)                                                                                                                      cTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTgg     <  1:293988/139‑1 (MQ=255)                                                                                                                         tgGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCg  >  2:152194/1‑139 (MQ=255)                                                                                                                         tgGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCg  >  1:30688/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                 TTTGCTGGCACAATCCTCTTATCAGCTTGTCGAAGTGCAAGCCTCTCTGGAAGAGTCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCG  >  NZ_CP009273/1149376‑1149634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑