Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,154,375	A→C	S351R (AGC→CGC)	ptsG →	PTS glucose transporter subunit IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,154,375	0	A	C	100.0%	102.9 / NA	29	S351R (AGC→CGC)	ptsG	PTS glucose transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/10);  total (19/10)

TCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATG  >  NZ_CP009273/1154262‑1154501                                                                                                                  |                                                                                                                               tcatcCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGAt                                                                                                       >  2:176211/1‑139 (MQ=255)  catcCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGAtc                                                                                                      >  1:280896/1‑139 (MQ=255)  catcCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGAtc                                                                                                      >  2:336589/1‑139 (MQ=255)  catcCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGAtc                                                                                                      >  1:332532/1‑139 (MQ=255)    tcCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGAtcgt                                                                                                    >  1:79064/1‑139 (MQ=255)    tcCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGAtcgt                                                                                                    >  2:105834/1‑139 (MQ=255)      cACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGAtcgtc                                                                                                   <  1:7873/138‑1 (MQ=255)        cgcgATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGATCGTCGGt                                                                                                <  1:336589/139‑1 (MQ=255)          ctaTTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCTCAGCAAACTGTGGCGGTTCCCGATCGTCGGTAt                                                                                              <  1:121713/137‑1 (MQ=255)              tCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGt                                                                                          >  1:383347/1‑139 (MQ=255)              tCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGt                                                                                          >  2:127043/1‑139 (MQ=255)                  gCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTa                                                                                        <  2:280896/137‑1 (MQ=255)                       ccTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGAtt                                                                                 >  2:291740/1‑139 (MQ=255)                                cccAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTtacta                                                                         >  1:244180/1‑138 (MQ=255)                                    aTCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCa                                                                    <  2:429038/139‑1 (MQ=255)                                                ctGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTta                                                                            >  1:49073/1‑119 (MQ=255)                                                ctGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTta                                                                            <  2:49073/119‑1 (MQ=255)                                                         cGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAg                                               <  2:293029/139‑1 (MQ=255)                                                             aCGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGAtcgt                                                                                                    >  1:298798/1‑82 (MQ=255)                                                             aCGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGAtcgt                                                                                                    <  2:298798/82‑1 (MQ=255)                                                                         tctcGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAAc                               >  1:439184/1‑139 (MQ=255)                                                                           tcGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGc                             >  2:102405/1‑139 (MQ=255)                                                                                             ttCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGa           >  2:49488/1‑139 (MQ=255)                                                                                             ttCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGa           >  2:330531/1‑139 (MQ=255)                                                                                             ttCATCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGa           >  1:313439/1‑139 (MQ=255)                                                                                                 tCGTTCTGTCTGGTAACCGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGa       <  1:330531/139‑1 (MQ=255)                                                                                                       tgtctgGTAACCGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATg  >  1:304676/1‑138 (MQ=255)                                                                                                         tctgGTAACCGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGa                <  2:182273/122‑1 (MQ=255)                                                                                                         tctgGTAACCGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGa                >  1:182273/1‑122 (MQ=255)                                                                                                                  |                                                                                                                               TCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATG  >  NZ_CP009273/1154262‑1154501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑