Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,285,718	A→G	G7G (GGA→GGG)	rssB →	two‑component system response regulator RssB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,285,718	0	A	G	100.0%	34.8 / NA	11	G7G (GGA→GGG)	rssB	two‑component system response regulator RssB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/6);  total (5/6)

GGTACGCACCAACATTTGACCAGAATTTTTATCTACACTTAAGTTAATTCTGACAGGCGCAGGTGGCAATAGCATGCCACTATTGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTT  >  NZ_CP009273/1285588‑1285845                                                                                                                                   |                                                                                                                                ggTACGCACCAACATTTGACCAGAATTTTTATCTACACTTAAGTTAATTCTGACAGGCGCAGGTGGCAATAGCATGCCACTATTGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGGAAACAGAt                                                                                                                         >  2:391888/1‑139 (MQ=255)                        aaTTTTTATCTACACTTAAGTTAATTCTGACAGGCGCAGGTGGCAATAGCATGCCACTATTGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGGAAACAGATTCTCATTGTTGAAGATGAGCAgg                                                                                                  >  2:269928/1‑139 (MQ=255)                             ttATCTACACTTAAGTTAATTCTGACAGGCGCAGGTGGCAATAGCATGCCACTATTGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGGAAACAGATTCTCATTGTTGAAGATGAGCAGGTAtt                                                                                              <  1:269928/138‑1 (MQ=255)                                    cacTTAAGTTAATTCTGACAGGCGCAGGTGGCAATAGCATGCCACTATTGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGGAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTtcgct                                                                                         <  2:232173/136‑1 (MQ=255)                                                     aCAGGCGCAGGTGGCAATAGCATGCCACTATTGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGGAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGttt                                                                     <  1:391888/139‑1 (MQ=255)                                                                           tGCCACTATTGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGGAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACgg                                               >  1:348731/1‑139 (MQ=255)                                                                                           aGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGGAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTtcgct                                                                                         <  2:399824/81‑1 (MQ=255)                                                                                           aGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGACGGGAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTtcgct                                                                                         >  1:399824/1‑81 (MQ=255)                                                                                             ccAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGGAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgggg                             >  2:385320/1‑139 (MQ=255)                                                                                                            aaCATGACGCAGCCATTGGTCGGGAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCGCGCTTCTGGATTCATGGTTTTCCGCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGt              <  1:421189/139‑1 (MQ=255)                                                                                                                        ccATTGGTCGGGAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGtt  <  2:348731/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                GGTACGCACCAACATTTGACCAGAATTTTTATCTACACTTAAGTTAATTCTGACAGGCGCAGGTGGCAATAGCATGCCACTATTGAGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTT  >  NZ_CP009273/1285588‑1285845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑