Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	204,632	T→C	V1007A (GTC→GCC)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	204,632	0	T	C	100.0%	98.0 / NA	29	V1007A (GTC→GCC)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/13);  total (16/13)

ACCGGACACCCTATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTAT  >  NZ_CP009273/204496‑204758                                                                                                                                         |                                                                                                                               aCCGGACACCCTATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCa                                                                                                                              >  2:123910/1‑139 (MQ=255)  ccGGACACCCTATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCAc                                                                                                                             >  2:106176/1‑139 (MQ=255)     gACACCCTATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCAcc                                                                                                                            >  2:399981/1‑137 (MQ=255)     gACACCCTATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCAcc                                                                                                                            >  1:411253/1‑137 (MQ=255)      acacCCTATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACTTTGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGTTTATGGCCACCAAg                                                                                                                         <  2:230200/139‑1 (MQ=255)       cacCCTATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAgc                                                                                                                        >  2:432530/1‑139 (MQ=255)              tCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCaa                                                                                                                 <  1:341886/139‑1 (MQ=255)                aaCCAGTAATTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAAt                                                                                                                <  1:390919/138‑1 (MQ=255)                     gTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATc                                                                                                          >  1:395012/1‑139 (MQ=255)                           aaaaGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATc                                                                                                    >  2:182623/1‑139 (MQ=255)                                  aTTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGc                                                                                             >  1:151438/1‑139 (MQ=255)                                  aTTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGc                                                                                             >  2:3672/1‑139 (MQ=255)                                     gAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTg                                                                                           <  2:151438/138‑1 (MQ=255)                                           tATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGAcc                                                                                    >  2:272546/1‑139 (MQ=255)                                             tGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGt                                                                                  >  1:361072/1‑139 (MQ=255)                                                        tAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTAt                                                                                                           <  1:324136/103‑1 (MQ=255)                                                        tAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTAt                                                                                                           >  2:324136/1‑103 (MQ=255)                                                                              gACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGAc                                                 >  1:200108/1‑139 (MQ=255)                                                                                  gAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGccc                                             <  1:318280/139‑1 (MQ=255)                                                                                  gAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGccc                                             <  1:123910/139‑1 (MQ=255)                                                                                                  tcaCGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGc                              >  1:259035/1‑138 (MQ=255)                                                                                                            ggggCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGaa                   <  2:186034/139‑1 (MQ=255)                                                                                                               gCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGaaaaa                <  2:192890/139‑1 (MQ=255)                                                                                                                   gttgttGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGAc             <  1:272546/138‑1 (MQ=255)                                                                                                                       ttgCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCa         <  2:361072/138‑1 (MQ=255)                                                                                                                             cgcgGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGa              >  2:232553/1‑127 (MQ=255)                                                                                                                             cgcgGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGa              <  1:232553/127‑1 (MQ=255)                                                                                                                             cgcgGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTAt  >  1:265603/1‑139 (MQ=255)                                                                                                                             cgcgGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTAt  <  1:106176/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                               ACCGGACACCCTATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTAT  >  NZ_CP009273/204496‑204758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑