| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,336,067 | G→A | intergenic (+83/‑111) | lapB → / → pyrF | lipopolysaccharide assembly protein LapB/orotidine‑5'‑phosphate decarboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,336,067 | 0 | G | A | 93.3% | 50.1 / ‑5.9 | 15 | intergenic (+83/‑111) | lapB/pyrF | lipopolysaccharide assembly protein LapB/orotidine‑5'‑phosphate decarboxylase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (5/9); total (5/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTCGCGCCACTTCCGGTGCCCATCATCAAGAAGGTCTGGTCATGACG > NZ_CP009273/1335961‑1336183 | aTTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAAttt > 2:196637/1‑139 (MQ=255) gcgGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTa < 1:265572/139‑1 (MQ=255) cTGTAATTTTTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAACTTTTTATTTCAACGGGTAg > 2:185121/1‑138 (MQ=255) tAATTTTTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAAt < 1:431498/138‑1 (MQ=255) aTTTTTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAAGGTTACATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCt < 1:191344/139‑1 (MQ=255) ttAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGc < 2:386634/138‑1 (MQ=255) acaTACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTCGCGCCACTTCCgg < 2:275827/139‑1 (MQ=255) cTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTCGCGCCACTTCCGGTGcc < 1:196637/138‑1 (MQ=255) attaATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTCGCGCCACTTCCGGTGCCcatcat > 2:197851/1‑138 (MQ=255) aaTGTCCCATTGTGCTCCGGCAAGGACGGCGCAAAAAAAGCCGGCAAGGGGAGAAATCGCAACGGTTAA‑TTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTCGCGCCACTTCCGGTGCCCATCATCaag < 1:301427/138‑1 (MQ=255) aTGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTCGCGCCACTTCCGGTGCCCATCATCaag < 2:371908/138‑1 (MQ=255) tgCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTCGCGCCACTTCCGGTGCCCATCATCAAGAAGGTCTGGTc < 2:155174/138‑1 (MQ=255) cTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCg < 1:310235/86‑1 (MQ=255) cTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCg > 2:310235/1‑86 (MQ=255) cGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTCGCGCCACTTCCGGTGCCCATCATCAAGAAGGTCTGGTCATGACg > 1:262825/1‑139 (MQ=255) aaaaaaCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTCGCGCCACTTCCGGTGCCCATCATCAAGAAGGTCTGGTCAt < 1:37080/118‑1 (MQ=255) aaaaaaCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTCGCGCCACTTCCGGTGCCCATCATCAAGAAGGTCTGGTCAt > 2:37080/1‑118 (MQ=255) | ATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTCGCGCCACTTCCGGTGCCCATCATCAAGAAGGTCTGGTCATGACG > NZ_CP009273/1335961‑1336183 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |