Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,449,980	A→G	D187G (GAT→GGT)	paaC →	phenylacetate‑CoA oxygenase subunit PaaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,449,980	0	A	G	100.0%	80.6 / NA	23	D187G (GAT→GGT)	paaC	phenylacetate‑CoA oxygenase subunit PaaC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/14);  total (9/14)

ACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATT‑‑GCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCT  >  NZ_CP009273/1449849‑1450106                                                                                                                                      |                                                                                                                               aCTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATT‑‑GCGGTTGGTCCACGc                                                                                                                         <  2:351539/139‑1 (MQ=255) aCTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGGGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATT‑‑GCGGTTGGTCCACGc                                                                                                                         <  2:230364/139‑1 (MQ=255)  cTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATT‑‑GCGGTTGGTCCACGCa                                                                                                                        >  1:370163/1‑139 (MQ=255)                                                 caacaaGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATT‑‑GCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACg                                                                           >  2:265644/1‑137 (MQ=255)                                                    caaGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATT‑‑GCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGcc                                                                      <  1:24679/139‑1 (MQ=255)                                                    caaGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATT‑‑GCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGcc                                                                      <  1:308560/139‑1 (MQ=255)                                                                            aCTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATT‑‑GCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAg                                              <  1:265644/139‑1 (MQ=255)                                                                                       ccGACGAGATTGATATTGCACTGAGTGAAGAGGGTAGT‑‑GCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGTCGTATCGCa                                    <  2:191808/138‑1 (MQ=255)                                                                                           cgaGATTGATATTGCACTGAGTGAAGAGGGTATT‑‑GCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGc                               <  1:404667/139‑1 (MQ=255)                                                                                               aTTGATATTGCACTGAGTGAAGAGGGTATT‑‑GCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGt                            >  2:5440/1‑138 (MQ=255)                                                                                               aTTGATATTGCACTGAGTGAAGAGGGTATT‑‑GCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTa                           <  1:5440/139‑1 (MQ=255)                                                                                                    tatTGCACTGAGTGAAGAGGGTATTGCGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTaaaa                        <  2:7460/139‑1 (MQ=255)                                                                                                         cACTGAGTGAAGAGGGTATT‑‑GCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACt                 >  1:96338/1‑139 (MQ=255)                                                                                                           cTGAGTGAAGAGGGTAGT‑‑GCGGTTGGGCCAAGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGc               <  2:405988/139‑1 (MQ=255)                                                                                                              aGTGAAGAGGGTATT‑‑GCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGCCTGCCTa            >  1:202234/1‑139 (MQ=255)                                                                                                                   agagGGTATT‑‑GCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGaa       <  1:89102/139‑1 (MQ=255)                                                                                                                   agagGGTATT‑‑GCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGaa       <  2:163156/139‑1 (MQ=255)                                                                                                                   agagGGTATT‑‑GCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGaa       >  1:218914/1‑139 (MQ=255)                                                                                                                        gTATT‑‑GCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCg                                           <  2:255025/98‑1 (MQ=255)                                                                                                                        gTATT‑‑GCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCg                                           >  1:255025/1‑98 (MQ=255)                                                                                                                        gTATT‑‑GCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATAACGAAAACCt  >  1:422868/1‑139 (MQ=255)                                                                                                                                   ttGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGaa       <  2:33824/125‑1 (MQ=255)                                                                                                                                   ttGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGaa       >  1:33824/1‑125 (MQ=255)                                                                                                                                      |                                                                                                                               ACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATT‑‑GCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCT  >  NZ_CP009273/1449849‑1450106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑