Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,492,661	A→G	M517V (ATG→GTG)	opgD →	glucan biosynthesis protein OpgD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,492,661	0	A	G	100.0%	88.7 / NA	25	M517V (ATG→GTG)	opgD	glucan biosynthesis protein OpgD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/13);  total (12/13)

CGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACC  >  NZ_CP009273/1492533‑1492787                                                                                                                                 |                                                                                                                               cGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACg                                                                                                                      <  1:249050/139‑1 (MQ=255)               tCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACg                                                                                                        >  2:185387/1‑139 (MQ=255)                ccAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACg                                                                                                        <  1:358333/138‑1 (MQ=255)                      gggAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAg                                                                                                 <  1:365720/139‑1 (MQ=255)                         aagcgaagcAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTg                                                                                               >  2:190544/1‑138 (MQ=255)                           gcgaagcAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGaaa                                                                                            >  1:254192/1‑139 (MQ=255)                           gcgaagcAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGaaa                                                                                            >  1:274428/1‑139 (MQ=255)                            cgaagcAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAAc                                                                                           <  2:366331/139‑1 (MQ=255)                                                       aaCCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAgc                                                                >  1:60728/1‑139 (MQ=255)                                                            aTCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCg                                                            >  1:388572/1‑138 (MQ=255)                                                            aTCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCg                                                            >  2:62862/1‑138 (MQ=255)                                                            aTCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCg                                                            >  1:120185/1‑138 (MQ=255)                                                                         gTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGt                                               <  1:378866/138‑1 (MQ=255)                                                                         gTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGt                                               <  1:217436/138‑1 (MQ=255)                                                                         gTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGt                                               <  2:254192/138‑1 (MQ=255)                                                                         gTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGt                                               <  2:274428/138‑1 (MQ=255)                                                                                 tttGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGt                                               >  1:294206/1‑130 (MQ=255)                                                                                 tttGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGt                                               <  2:294206/130‑1 (MQ=255)                                                                                         gTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGtg                              <  2:388572/139‑1 (MQ=255)                                                                                                           cACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGt                                                   >  2:131095/1‑100 (MQ=255)                                                                                                           cACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGt                                                   <  1:131095/100‑1 (MQ=255)                                                                                                                aTCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCg       <  1:87006/139‑1 (MQ=255)                                                                                                                     gTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGtg                              >  2:160353/1‑111 (MQ=255)                                                                                                                     gTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGtg                              <  1:160353/111‑1 (MQ=255)                                                                                                                     gTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGAAcc  >  1:355129/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                               CGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATTCTCTATATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACC  >  NZ_CP009273/1492533‑1492787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑