Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,528,129	G→T	intergenic (‑21/‑152)	yddH ← / → nhoA	flavin reductase family protein/N‑hydroxyarylamine O‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,528,129	0	G	T	100.0%	91.7 / NA	23	intergenic (‑21/‑152)	yddH/nhoA	flavin reductase family protein/N‑hydroxyarylamine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/9);  total (14/9)

AGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTAA‑CGCAATAAGTAACGAAATTAACGGGATTGGCGATTTGCGAACGTGATGCATGTCCGCGATCGCACAAAATAGCCGGTGCGGCGTCTATTCCAGGTTATAA  >  NZ_CP009273/1527995‑1528251                                                                                                                                       |                                                                                                                            aGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGTtttt                                                                                                                         >  1:290357/1‑139 (MQ=255)           ttACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGTTTTTTTTAAGCGTa                                                                                                               <  2:128491/139‑1 (MQ=255)             aCGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGt                                                                                                             >  1:400468/1‑139 (MQ=255)             aCGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGt                                                                                                             >  1:340562/1‑139 (MQ=255)                  gcTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGTCCGTa                                                                                                        <  2:342065/139‑1 (MQ=255)                   cTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGTCCGTaa                                                                                                       >  1:349711/1‑139 (MQ=255)                   cTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGTCCGTaa                                                                                                       >  1:160199/1‑139 (MQ=255)                            cTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGTCCGTAA‑CGCAATAAg                                                                                             >  2:339726/1‑139 (MQ=255)                                      cTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGTCCGTAACCGCAATAAGTAACGAAAt                                                                                    >  1:380839/1‑139 (MQ=255)                                                          gCCATGATTTAACAGACGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGTCCGTAACCGCAATAAGTAACGAAATTAACGGGATTGGCGATTTGc                                                                <  2:380839/139‑1 (MQ=255)                                                                           ggCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGTCCGTAA‑CGCAATAAGTAACGAAATTAACGGGATTGGCGATTTGCGAACGTGATGCATGTCcg                                              >  2:28162/1‑139 (MQ=255)                                                                                 cATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGTCCGTAA‑CGCAATAAGTAACGAAATTAACGGGATTGGCGATTTGCGAACGTGATGCATGTCCGCGATCg                                        <  2:290357/139‑1 (MQ=255)                                                                                          aTTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGTCCGTAA‑CGCAATAAGTAACGAAATTAACGGGATTGGCGATTTGCGAACGTGATGCATGTCCGCGATCGCACaaaa                                 >  1:428521/1‑137 (MQ=255)                                                                                           ttCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGTCCGTAA‑CGCAATAAGTAACGAAATTAACGGGATTGGCGATTTGCGAACGTGATGCATGTCCGCGATCGCACAAAATAg                              >  2:213510/1‑139 (MQ=255)                                                                                                tGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGTCCGTAA‑CGCAATAAGTAACGAAATTAACGGGATTGGCGATTTGCGa                                                              <  1:347965/102‑1 (MQ=255)                                                                                                tGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGTCCGTAA‑CGCAATAAGTAACGAAATTAACGGGATTGGCGATTTGCGa                                                              >  2:347965/1‑102 (MQ=255)                                                                                                tGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGTCCGTAA‑CGCAATAAGTAACGAAATTAACGGGATTGGCGATTTGCGAACGTGATGCATGTCCGCGATCGCACAAAATAGCCGGt                         >  1:356312/1‑139 (MQ=255)                                                                                                   ggATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGTCCGTAA‑CGCAATAAGTAACGAAATTAACGGGATTGGCGATTTGCGAACGTGATGCATGTCCGCGATCGCACAAAATAGCCGGTgcg                      <  1:339726/139‑1 (MQ=255)                                                                                                     aTGAATCGGCTCATGGTATCGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGTCCGTAA‑CGCAATAAGTAACGAAATTAACGGGATTGGCGATTTGCGAACGTGATGCATGTCCGCGATCGCACAAAATAGCCGGTgcggc                    >  1:86842/1‑139 (MQ=255)                                                                                                                     tATCGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGTCCGTAA‑CGCAATAAGTAACGAAATTAACGGGATTGGCGATTTGCGAACGTGATGCATGTCCGCGATCGCACAAAATAGCCGGTGCGGCGTCTATTCCAGGTtat    <  2:400468/139‑1 (MQ=255)                                                                                                                       tCGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGTCCGTAA‑CGCAATAAGTAACGAAATTAACGGGATTGGCGATTTGCGAACGTGATGCATGTCCGCGATCGCACAAAATAGCCGGTGCGGCGTCTATTCCAGGTTATaa  <  1:170891/139‑1 (MQ=255)                                                                                                                        cGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGTCCGTAA‑CGCAATAAGTAACGAAATTAACGGGATTGGCGATTTGCGAACGTGATGCATGTCCGCGATCGCACAAAATAGcc                            >  2:198384/1‑112 (MQ=255)                                                                                                                        cGCCTCGCGAAGAAGTTTTTTTTAAGCGTAGTCCGTAA‑CGCAATAAGTAACGAAATTAACGGGATTGGCGATTTGCGAACGTGATGCATGTCCGCGATCGCACAAAATAGcc                            <  1:198384/112‑1 (MQ=255)                                                                                                                                       |                                                                                                                            AGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACGGCTGGCATGGTGTAATTCTATGGGGATGAATCGGCTCATGGTATCGCCTCGCGAAGAAGGTTTTTTTAAGCGTAGTCCGTAA‑CGCAATAAGTAACGAAATTAACGGGATTGGCGATTTGCGAACGTGATGCATGTCCGCGATCGCACAAAATAGCCGGTGCGGCGTCTATTCCAGGTTATAA  >  NZ_CP009273/1527995‑1528251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑