Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,542,720	A→G	T355A (ACT→GCT)	fdnG →	formate dehydrogenase‑N subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,542,720	0	A	G	100.0%	40.8 / NA	12	T355A (ACT→GCT)	fdnG	formate dehydrogenase‑N subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/10);  total (2/10)

CGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGAAAGTGTGTG  >  NZ_CP009273/1542594‑1542831                                                                                                                               |                                                                                                                cGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCt                                                                                                     <  1:358528/139‑1 (MQ=255) cGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCt                                                                                                     <  1:360693/139‑1 (MQ=255) cGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCt                                                                                                     <  1:367703/139‑1 (MQ=255) cGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCt                                                                                                     <  2:299114/139‑1 (MQ=255)                                            acgCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGcc                                                          <  2:129069/139‑1 (MQ=255)                                                  aaaaaCGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGAcg                                                     <  1:161318/138‑1 (MQ=255)                                                    aaaCGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGAcgtcg                                                  <  2:14804/139‑1 (MQ=255)                                                                           cTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGcc                            <  2:194969/138‑1 (MQ=255)                                                                                    tCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGa                   <  2:23928/138‑1 (MQ=255)                                                                                    tCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGAc                  >  1:118635/1‑139 (MQ=255)                                                                                        cTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTcc              >  2:44021/1‑139 (MQ=255)                                                                                                    ggCTATGCGAAACGCGATGAAACACTGGCTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGAAAGtgtgtg  <  1:156361/139‑1 (MQ=255)                                                                                                                               |                                                                                                                CGTGATGATTTTGCTTTCGAAGACGGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTATCAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTGTGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGCGGTACGCCAAAAGCCGACTTCCTGAAAGTGTGTG  >  NZ_CP009273/1542594‑1542831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑