Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,555,636	T→C	N368S (AAC→AGC)	ddpA ←	ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,555,636	0	T	C	100.0%	87.6 / NA	26	N368S (AAC→AGC)	ddpA	ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/12);  total (14/12)

ACCTTTACCCACTCTGTCGCGCATGGTGGCGTTCGCCAGCTTTTCCAGCTTCACAATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCGGCTTTGGCTTTCGTTTCGTCATGGTTGTATTGCATTGCCGTCGCATCGTAGCCCCACATGCCTTCCG  >  NZ_CP009273/1555527‑1555768                                                                                                              |                                                                                                                                     aCCTTTACCCACTCTGTCGCGCATGGTGGCGTTCGCCAGCTTTTCCAGCTTCACAATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGt                                                                                                          >  2:199779/1‑138 (MQ=255)   cTTTACCCACTCTGTCGCGCATGGTGGCGTTCGCCAGCTTTTCCAGCTTCACAATGCTGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAg                                                                                                       >  1:165673/1‑139 (MQ=255)      tACCCACTCTGTCGCGCATGGTGGCGTTCGCCAGCTTTTCCAGCTTCACAATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCt                                                                                                    >  1:194632/1‑139 (MQ=255)      tACCCACTCTGTCGCGCATGGTGGCGTTCGCCAGCTTTTCCAGCTTCACAATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCt                                                                                                    >  1:283065/1‑139 (MQ=255)             tctGTCGCGCATGGTGGCGTTCGCCAGCTTTTCCAGCTTCACAATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTAt                                                                                                                          >  1:219368/1‑110 (MQ=255)             tctGTCGCGCATGGTGGCGTTCGCCAGCTTTTCCAGCTTCACAATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTAt                                                                                                                          <  2:219368/110‑1 (MQ=255)             tctGTCGCGCATGGTGGCGTTCGCCAGCTTTTCCAGCTTCACAATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGt                                                                                             >  2:75283/1‑139 (MQ=255)             tctGTCGCGCATGGTGGCGTTCGCCAGCTTTTCCAGCTTCACAATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGt                                                                                             >  1:101464/1‑139 (MQ=255)                    cgcATGGTGGCGTTCGCCAGCTTTTCCAGCTTCACAATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCg                                                                                      <  2:101464/139‑1 (MQ=255)                      cATGGTGGCGTTCGCCAGCTTTTCCAGCTTCACAATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTAt                                                                                                                          <  1:174064/101‑1 (MQ=255)                      cATGGTGGCGTTCGCCAGCTTTTCCAGCTTCACAATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTAt                                                                                                                          >  2:174064/1‑101 (MQ=255)                      cATGGTGGCGTTCGCCAGCTTTTCCAGCTTCACAATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCGt                                                                                     >  2:424388/1‑138 (MQ=255)                       aTGGTGGCGTTCGCCAGCTTTTCCAGCTTCACAATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCa                                                                                   <  1:314432/139‑1 (MQ=255)                                        cTTTTCCAGCTTCACAATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCggct                                                                  <  2:194632/139‑1 (MQ=255)                                                    cacaATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCCCTTTATCCCATTCGGCTTTGGCtttcgt                                                       <  1:65410/138‑1 (MQ=255)                                                     acaATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCGGCTTTGGCtttcgtt                                                      <  1:75283/138‑1 (MQ=255)                                                              cccAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCGGCTTTGGCTTTCGTTTCGTCATgg                                             <  2:165673/138‑1 (MQ=255)                                                              cccAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCGGCTTTGGCTTTCGTTTCGTCATGGt                                            >  2:256422/1‑139 (MQ=255)                                                              cacAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCGGCTTTGGCTTTCGTTTCGTCATgg                                             <  1:177271/136‑1 (MQ=255)                                                                        tgAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCGGCTTTGGCTTTCGTTTCGTCATGGTTGTattgcat                                  >  1:368107/1‑139 (MQ=255)                                                                                gATTGTGTCGCCAGAGCAATAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCGGCTTTGGCTTTCGTTTCGTCATGGTTGTATTGCATTGCCGTCg                          <  1:47728/139‑1 (MQ=255)                                                                                                 aaTAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCGGCTTTGGCTTTCGTTTCGTCATGGTTGTATTGCATTGCCGTCGCATCGTAGCCCCACATg         <  2:368107/139‑1 (MQ=255)                                                                                                   tAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCGGCTTTGGCTTTCGTTTCGTCATGGTTGTATTGCATTGCCGTCGCATCg                     <  1:407470/125‑1 (MQ=255)                                                                                                   tAGGCTCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCGGCTTTGGCTTTCGTTTCGTCATGGTTGTATTGCATTGCCGTCGCATCg                     >  2:407470/1‑125 (MQ=255)                                                                                                        tCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCGGCTTTGGCTTTCGTTTCGTCATGGTTGTATTGCATTGCCGTCGCATCGTAGCCCCACATGCCTTCCg  >  2:363595/1‑139 (MQ=255)                                                                                                        tCCCAGCTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCGGCTTTGGCTTTCGTTTCGTCATGGTTGTATTGCATTGCCGTCGCATCGTAGCCCCACATGCCTTCCg  >  1:153436/1‑139 (MQ=255)                                                                                                              |                                                                                                                                     ACCTTTACCCACTCTGTCGCGCATGGTGGCGTTCGCCAGCTTTTCCAGCTTCACAATGATGCCCAGCTTGTTGAGACTGGATTGTGTCGCCAGAGCAATAGGCTCCCAGTTCGGATCGTTATCGGAGTAGAGAAACGTCAGGCTGGTGGGTTTGCTCGTCACTTTATCCCATTCGGCTTTGGCTTTCGTTTCGTCATGGTTGTATTGCATTGCCGTCGCATCGTAGCCCCACATGCCTTCCG  >  NZ_CP009273/1555527‑1555768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑