| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,575,730 | T→C | D351G (GAT→GGT) | ydeN ← | sulfatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,575,730 | 0 | T | C | 88.9% | 44.6 / 0.1 | 18 | D351G (GAT→GGT) | ydeN | sulfatase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (8/8); total (9/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
GTTTTCCTTTCCACCACATAAACATTGGGGTGTGAGTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCATCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGATCAACAGAATAAACGGAAGCGTAGTAGTTATCTGC > NZ_CP009273/1575632‑1575860 | gTTTTCCTTTCCACCACATAAACATTGGGGTGTGAGTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAattgt < 1:140822/139‑1 (MQ=255) gggTGTGAGTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAg > 1:141762/1‑139 (MQ=255) gggTGTGAGTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAg > 1:73038/1‑139 (MQ=255) tgAGTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAgttgtt > 1:328292/1‑139 (MQ=255) tgAGTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCACGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTGCAgttgtt < 2:86094/139‑1 (MQ=255) cGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAAt < 2:141762/139‑1 (MQ=255) cAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCg > 2:237032/1‑139 (MQ=255) ggTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTaca < 2:424299/139‑1 (MQ=255) gTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTacac > 1:365501/1‑139 (MQ=255) tCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACAcc < 2:317814/139‑1 (MQ=255) tttGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAattg < 2:295113/69‑1 (MQ=255) tttGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAattg > 1:295113/1‑69 (MQ=255) gcgcCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGATCAACAGAATAAACgg < 1:352270/138‑1 (MQ=255) cccGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGATCAACAGAATAAACGGAAGCg < 1:93583/139‑1 (MQ=255) gagGACCATCGATAACCGCACCATTATCGGAGGTAAAGAGAATAattg < 1:733/48‑1 (MQ=255) gagGACCATCGATAACCGCACCATTATCGGAGGTAAAGAGAATAattg > 2:733/1‑48 (MQ=255) gagGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGATCAACAGAATAAACGGAAGCGTAGTAGTTATctgc > 2:287858/1‑139 (MQ=255) gagGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGATCAACAGAATAAACGGAAGCGTAGTAGTTATctgc > 2:83872/1‑139 (MQ=255) | GTTTTCCTTTCCACCACATAAACATTGGGGTGTGAGTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCATCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGATCAACAGAATAAACGGAAGCGTAGTAGTTATCTGC > NZ_CP009273/1575632‑1575860 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |