Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,578,845	A→G	D633D (GAT→GAC)	ydeP ←	acid resistance putative oxidoreductase YdeP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,578,845	0	A	G	100.0%	103.1 / NA	29	D633D (GAT→GAC)	ydeP	acid resistance putative oxidoreductase YdeP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/12);  total (17/12)

ATAAAGACCACATCTCGTTGACCGAATACCCCTCGATAGCGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGATCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATGCG  >  NZ_CP009273/1578717‑1578955                                                                                                                                 |                                                                                                               aTAAAGACCACATCTCGTTGACCGAATACCCCTCGATAGCGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTaa                                                                                                        >  2:19975/1‑137 (MQ=255)           caTCTCGTTGACCGAATACCCCTCGATAGCGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTaa                                                                                                        <  2:11124/127‑1 (MQ=255)           caTCTCGTTGACCGAATACCCCTCGATAGCGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTaa                                                                                                        >  1:11124/1‑127 (MQ=255)           caTCTCGTTGACCGAATACCCCTCGATAGCGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGc                                                                                             <  2:305218/138‑1 (MQ=255)           caTCTCGTTGACCGAATACCCCTCGATAGCGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGc                                                                                             >  1:305218/1‑138 (MQ=255)                                cTCGATAGCGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCtt                                                                                                >  1:284851/1‑114 (MQ=255)                                cTCGATAGCGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCtt                                                                                                <  2:284851/114‑1 (MQ=255)                                cTCGATAGCGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTAcc                                                                       >  2:368675/1‑139 (MQ=255)                                        cGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCg                                                               <  1:19975/139‑1 (MQ=255)                                                  taccataAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAgcg                                                     >  2:304155/1‑139 (MQ=255)                                                       taAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCttt                                                >  2:424805/1‑139 (MQ=255)                                                             gtcgtGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTcagcag                                          <  1:304155/139‑1 (MQ=255)                                                                        cTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTAt                                <  1:116605/138‑1 (MQ=255)                                                                        cTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATc                               <  1:78301/139‑1 (MQ=255)                                                                           aTCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAgg                            >  2:309840/1‑139 (MQ=255)                                                                                ggCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGaa                        >  1:65038/1‑138 (MQ=255)                                                                                ggCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGaa                        >  1:331153/1‑138 (MQ=255)                                                                                ggCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGaa                        >  1:292788/1‑138 (MQ=255)                                                                                ggCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGaa                        >  1:185644/1‑138 (MQ=255)                                                                                     cGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCCTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTcagcag                                          >  1:60836/1‑115 (MQ=255)                                                                                     cGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCCTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTcagcag                                          <  2:60836/115‑1 (MQ=255)                                                                                          tGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGa             >  2:226573/1‑139 (MQ=255)                                                                                               ccATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACg        >  1:195122/1‑139 (MQ=255)                                                                                                     ccAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATgcg  <  1:226573/139‑1 (MQ=255)                                                                                                     ccAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATgcg  <  2:62626/139‑1 (MQ=255)                                                                                                                   aaCGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCCCCGGGATGACGGATgcg  <  1:190002/125‑1 (MQ=255)                                                                                                                   aaCGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATgcg  >  2:190002/1‑125 (MQ=255)                                                                                                                   aaCGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATgcg  >  1:385516/1‑125 (MQ=255)                                                                                                                   aaCGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATgcg  <  2:385516/125‑1 (MQ=255)                                                                                                                                 |                                                                                                               ATAAAGACCACATCTCGTTGACCGAATACCCCTCGATAGCGATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGATCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATGCG  >  NZ_CP009273/1578717‑1578955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑