| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,520,009 | 0 | T | A | 28.6% | 59.1 / 11.7 | 28 | I77F (ATT→TTT) | ansP | L‑asparagine permease |
| Reads supporting (aligned to +/- strand): ref base T (16/4); new base A (0/8); total (16/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.59e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGC > NZ_CP009273/1519940‑1520084 | cACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCcaca > 2:414567/1‑91 (MQ=255)cACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCcaca > 2:522254/1‑91 (MQ=255) cGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACaaa > 1:386387/1‑91 (MQ=255) gggCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAAAAAAAAACAAAAAAAAGCCACAAAt < 1:745179/91‑1 (MQ=255) gggCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAAAATAAAAAACGAAAACAAGCCACAAAt < 2:46344/91‑1 (MQ=255) gggCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAAAAAAAAAAACAAAAACAAGCCACAAAt < 1:455994/91‑1 (MQ=255) cATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATtaa > 2:745179/1‑91 (MQ=255) aaaaaaaCAAAACTGCCACGGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAAAAAAAAAAACAAAAACAAGCCACAAATtaaat < 1:833212/87‑1 (MQ=255) aaaaaaCAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAAAAAAAAACAAAAACAAGCCACAAATtaaata < 1:736208/88‑1 (MQ=255) gCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAAccagtgccagt > 1:246199/1‑91 (MQ=255) ctgggAGGGGCGGTGTAGCCCCACCCCCCCAAAGGCAGGAAAAAAAAAAAACAAAAAAAAGCCACAAATTAAATAAACCAGTGCCAGTGCg < 1:414567/88‑1 (MQ=255) aCTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCg > 1:306004/1‑91 (MQ=255) gaGGGCGGTGTACCCCCAGCCCCCCAAATGCACGAAAAAAAAAAAACAAAAAAAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGccc < 1:75728/90‑1 (MQ=255) aaGGGCGTTGTACCCCCAGCTCCCCCAATGCCCGAAGAAAAAAAAACAAAAAAAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGccc < 1:341067/91‑1 (MQ=255) cggCGGGGAAGCCCCGGCCCAACAAATGCAGGAAGAAAAAAAAACGAAAAAAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCg < 1:489583/90‑1 (MQ=255) ggCGGGTTAGCCCCAGCCCCCCAAATGCACGAAAAAAAAAAAACAAAAAAAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGc < 1:182834/91‑1 (MQ=255) ggTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCAt > 1:789566/1‑91 (MQ=255) tAGCACCGGCTCACCCAATGCACGCAAAAAAAAAAGCGAAAAAAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGc < 2:420058/91‑1 (MQ=255) aCCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTc > 1:94734/1‑91 (MQ=255) aGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAAcc > 1:501043/1‑57 (MQ=255) aGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAAcc < 2:501043/57‑1 (MQ=255) aGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCTGCCATTTGCAGTCggg > 2:604984/1‑91 (MQ=255) aGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCggg > 1:157668/1‑91 (MQ=255) aGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCggg > 2:100801/1‑91 (MQ=255) aGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCggg > 1:947734/1‑91 (MQ=255) aGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCggg > 1:420058/1‑91 (MQ=255) caaaTGCACACAAAAAAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGc < 1:259444/89‑1 (MQ=255) aTGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAAcc < 2:1018296/47‑1 (MQ=255) aTGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAAcc > 1:1018296/1‑47 (MQ=255) aCGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAAccagtgc > 1:820204/1‑48 (MQ=255) aCGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAAccagtgc < 2:820204/48‑1 (MQ=255) | CACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGC > NZ_CP009273/1519940‑1520084 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |