| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,903 | T→A | D27E (GAT→GAA) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,903 | 0 | T | A | 100.0% | 147.8 / NA | 40 | D27E (GAT→GAA) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (18/22); total (18/22) | |||||||||||
CAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGC > NZ_CP009273/49821‑49985 | cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGccgg < 1:938737/91‑3 (MQ=255) aTCAGTCTGATTGCGGCTTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGGACTCGCCGGGTTTa < 2:359989/91‑1 (MQ=255) tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTaa > 2:807216/1‑91 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGcc < 1:11656/81‑1 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGcc > 2:11656/1‑81 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAAc > 1:290661/1‑91 (MQ=255) tGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCaa < 2:160077/91‑1 (MQ=255) tGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCaa < 2:19312/91‑1 (MQ=255) tGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCaa < 2:404042/91‑1 (MQ=255) tGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCaa < 1:1027571/91‑1 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCaa > 2:418270/1‑84 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCaa < 1:418270/84‑1 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTta > 1:619079/1‑91 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTta > 2:830463/1‑91 (MQ=255) cGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAAcc < 1:807216/91‑1 (MQ=255) gTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCg < 1:830463/91‑1 (MQ=255) tAGGTCGCGTTTTCGGCATGGAAAACCCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGt < 1:726332/91‑1 (MQ=255) tAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCaa < 2:408608/73‑1 (MQ=255) tAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCaa > 1:408608/1‑73 (MQ=255) tAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGt < 1:806875/91‑1 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAac < 2:971641/68‑1 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAac > 1:971641/1‑68 (MQ=255) cgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATg > 1:83429/1‑91 (MQ=255) ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg < 2:124390/91‑1 (MQ=255) tCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTaa < 1:259203/55‑1 (MQ=255) tCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTaa > 2:259203/1‑55 (MQ=255) cATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt > 2:714421/1‑91 (MQ=255) gAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt < 2:616018/91‑1 (MQ=255) aaaaCGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTg < 2:290661/91‑1 (MQ=255) cGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTa < 1:202739/41‑1 (MQ=38) cGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTa > 2:202739/1‑41 (MQ=38) cGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaa > 2:898496/1‑91 (MQ=255) tGGAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGCGATTATGGGCCGCCATACCTGGGAATCAATCGgtc > 2:327802/1‑91 (MQ=255) ggAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTtaaataa > 1:573161/1‑49 (MQ=38) ggAACCTGCCTGCCGAACTCGCCGGGTTTAAACGCAACACCTtaaataa < 2:573161/49‑1 (MQ=39) gcctgccGAACTCGCCGGGTTTAAACGCAACACCt > 1:838901/1‑35 (MQ=37) gcctgccGAACTCGCCGGGTTTAAACGCAACACCt < 2:401068/35‑1 (MQ=37) gcctgccGAACTCGCCGGGTTTAAACGCAACACCt > 1:401068/1‑35 (MQ=25) gcctgccGAACTCGCCGGGTTTAAACGCAACACCt < 2:838901/35‑1 (MQ=37) cctgccGAACTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGACGTCCGTTGc > 2:1004123/1‑91 (MQ=255) | CAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGC > NZ_CP009273/49821‑49985 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |