| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,453,604 | 0 | C | A | 47.1% | 12.2 / 13.3 | 17 | V98V (GTC→GTA) | paaC | 3‑hydroxyacyl‑CoA dehydrogenase PaaC |
| Reads supporting (aligned to +/- strand): ref base C (6/3); new base A (0/8); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.05e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGATCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAGCGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGATTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGC > NZ_CP009273/1453474‑1453731 | gcgTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGATCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAgc < 2:477287/139‑1 (MQ=255) tGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGATCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAGCGct < 1:504139/139‑1 (MQ=255) gggAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGATCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAGCGCTCTTTGca < 1:283810/139‑1 (MQ=255) cGCACATGGACACCCCTGCCCCGGGGGACCTATTTCCCCGCGCTGGCGGCGGCGGACCTGGCAAGTGAAGCGGCGTCTGAACTGCGGAAAGAAAAAAAGGCCCTCTCGGCACGGCGGGGGGAAGTTTCCCCGCCACaaa < 2:434067/139‑1 (MQ=255) aaccGCCTGCCCCGGGTCACCAATATCCCCGCGCGGGCAGCTGCGGCCCGGGTTATGGAGGCGGCGTCTGAACGTCGGGAAAAAAAAAAAGCGCTTTTTCCACAGCTGGGGGATGTTTGCCCGCCACAAACGCTATTGa < 2:659702/136‑1 (MQ=255) aaccGCCTGACCCCGGTGCCCGATATCCCCCCGCTGGCACCTGCGCCCCTGGTCATTGAGGCGGCCTCTGACCGTCTGAAAGCAAAAAACGCCCTTTTCCCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTg < 1:374861/135‑1 (MQ=255) cTGATCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAGCGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACt > 2:374861/1‑138 (MQ=255) gACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAGCGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTc > 1:550403/1‑138 (MQ=255) ccaatatCCCCGCGCGGGGAGCGGCGGCCCTGGTCATTGGGGGGGGGTCTGAAGGTCGGAAAAAAAAAAACCCTCTCTTTGCACAGGTGGCGGATTTTTCCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAAt < 2:483402/136‑1 (MQ=255) tatCCACGCGGGGGCGGCGGCGGCCGGGCTCTTTGCGGCGGCTTCTGAACGTCGGAAAGAAAAAAAGCCGCTCTTTACACAGCGGGGGGAATTTGCCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATctct < 2:318866/139‑1 (MQ=255) aCGCGGGGGCAGCGGCGGCCCTGGTCATAGAGGGGGCGTCTGACCGTCAGAAAGAAAAAAAAGCGCTCTTCGCCCGGCGGGGGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAAc < 2:67996/139‑1 (MQ=255) cTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAGCGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGa > 2:487105/1‑139 (MQ=255) cTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAGCGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGa > 2:508878/1‑139 (MQ=255) cTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAGCGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGa > 1:508113/1‑139 (MQ=255) aaGCGGCGTCTGAACGTCTGGAAGTCAAAAAAGCGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGATTGCTGCGGAGATAAAAAATCCTg > 1:366403/1‑138 (MQ=255) ggcggcgTCTAAACGTGTGAAAGTAAAAAAAGCGCTTTTCGCACAGCTGGGGAATGTTTCCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGATTGCTGCGGAGATAAAAAATCCTGaa < 1:575537/138‑1 (MQ=255) cttCTGAAAGAAAAAAAAGCTCTCTTCCCACAGCGGGCGGAATTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGATTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGc < 2:235212/137‑1 (MQ=255) | GCGTGACGCGGGGAAAACTGACTGCTGAAACCTGTGAACGCACATTGAAACGCCTGATCCCGGTGACCGATATTCACGCGCTGGCAGCTGCGGACCTGGTCATTGAAGCGGCGTCTGAACGTCTGGAAGTCAAAAAAGCGCTCTTTGCACAGCTGGCGGAAGTTTGCCCGCCACAAACGCTATTGACCACTAACACTTCGTCAATCTCTATAACCGCGATTGCTGCGGAGATAAAAAATCCTGAACGTGTTGCGGGGC > NZ_CP009273/1453474‑1453731 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |