| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,617,072 | 0 | C | A | 54.5% | 0.4 / 13.1 | 11 | G25G (GGC→GGA) | mgtT | protein MgtT |
| Reads supporting (aligned to +/- strand): ref base C (5/0); new base A (0/6); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCTGGCCGCGTATTTCAGCCACAAAT‑GGGATGACTAATGAACGGAGATAATCCCTCACCTAA‑CCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGT > NZ_CP009273/1616961‑1617206 | ttctggCGGTGT‑TTTCCACCAAAAATGGGGATAATAAATGAACGGAGA‑AATCCCCCACCAAACCCGGCCCT‑TTTTTATGGTGGTTAAAAGGGGGCT‑TGTTTTTTGGGCGGGAAAAAAAAAACCGCCAGTAAACcggcg < 1:428383/138‑1 (MQ=255) gAACGGAGATAATCCCTCACCTAA‑CCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACg > 1:501273/1‑139 (MQ=255) acccccTCCCCTAC‑CCGCCCCT‑TTTTTAAGGTTGTGAAAAAGGGGCT‑TGTTTTTTTGGCCGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCa < 2:199831/136‑1 (MQ=255) aaccccTCCCCTAC‑CCGCCCCT‑TTTTTACAGTTGT‑TAAAAGGGGCCTTGTTTTTTATGAGGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCa < 1:168561/136‑1 (MQ=255) aaccccACACCAAA‑CCGGCCCT‑TTTTAACTTTTGTCAAAAGGGTGCT‑TGATTTTTAGGAGGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCa < 2:448702/136‑1 (MQ=255) aTCCCCCCCCCAA‑CCCGCCCCTTTGTTACAGTGTGGTACGGGGGTCT‑TTTTTTTAATGAGGCAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCa < 2:404178/139‑1 (MQ=255) caacccGGCCCT‑TTGTTACGTTTGTGAACAAGGGGTG‑TTATTTTTATGACGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCa < 2:274455/135‑1 (MQ=255) cccc‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATa > 2:283600/1‑137 (MQ=255) ggCC‑TGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGt > 1:349613/1‑139 (MQ=255) gCC‑TGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGtt > 1:25070/1‑139 (MQ=255) cGGCGAAAAAAAACTGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGt > 1:25177/1‑138 (MQ=255) | TTCTGGCCGCGTATTTCAGCCACAAAT‑GGGATGACTAATGAACGGAGATAATCCCTCACCTAA‑CCGGCCCC‑TTGTTACAGTTGTGTACAAGGGGCC‑TGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGT > NZ_CP009273/1616961‑1617206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |